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Literature summary extracted from

  • Komoto, J.; Yamada, T.; Watanabe, K.; Woodward, D.F.; Takusagawa, F.
    Prostaglandin F2alpha formation from prostaglandin H2 by prostaglandin F synthase (PGFS): crystal structure of PGFS containing bimatoprost (2006), Biochemistry, 45, 1987-1996.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expressed in Escherichia coli HB101 cells Homo sapiens
1.1.1.188 expression in Escherichia coli strain HB101 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.188 in complex with bimatoprost and NADPH, hanging drop vapor diffusion method, using 1.0 mM BMP, 1.0 mM NADPH, 0.14 M NaCl, 50 mM MES buffer (pH 7.0), and 26% (w/v) PEG 8000 Homo sapiens
1.1.1.188 purified recombinant enzyme in complex with NADPH and bimatoprost BMP, an ocular hypotensive agent bound near the PGD2 binding site located on the alpha- and omega-chains, hanging drop vapour diffusion method, from 50 mM MES, pH 7.0, containing 7 mg/ml protein, 0.14 M NaCl, 26% w/v PEG 8000, 1.0 mM NADPH, and 1.0 mM BMP, added in a 95% ethanol solution, 4°C, 14 days, thick plate-shaped crystals, X-ray diffraction structure determination and analysis at 2.0 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.188 Y55F site-directed mutagenesis, the mutant loses its PGD2 11-ketoreductase activity, but not the PGH2 9,11-endoperoxide reductase Homo sapiens
1.1.1.188 Y55F the mutated enzyme loses the prostaglandin D2 11-ketoreductase activity but retains the prostaglandin H2 9,-11-endoperoxide reductase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.188 9,10-phenanthrenequinone the prostaglandin D2 11-ketoreductase activity of PGFS is competitively inhibited by 9,10-phenanthrenequinone Homo sapiens
1.1.1.188 bimatoprost inhibits the prostaglandin D2 11-ketoreductase activity as well as the prostaglandin H2 9,11-endoperoxide reductase activity of PGFS Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.188 additional information Homo sapiens the enzyme has dual activity as PGD2 11-ketoreductase and PGH2 9,11-endoperoxide reductase ?
-
 ?
1.1.1.188 prostaglandin D2 + NADPH + H+ Homo sapiens
-
 9alpha,11beta-prostaglandin F2alphabeta + NADP+
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+ Homo sapiens
-
 9alpha,11alpha-prostaglandin F2alpha + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Homo sapiens
-
-
-
1.1.1.188 Homo sapiens P42330
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188
-
 Homo sapiens
1.1.1.188 recombinant enzyme from Escherichia coli strain HB101 to homogeneity Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.188 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+ catalytic mechanisms, the catalytic sites for PGD2 11-ketoreductase and PGH2 9,11-endoperoxide reductase activities are located differently in the active site cavity, residue Y55 is important for the PGD2 11-ketoreductase activity, but not for the PGH2 9,11-endoperoxide reductase activity, involved structural features, overview Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 lung
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 additional information the enzyme has dual activity as PGD2 11-ketoreductase and PGH2 9,11-endoperoxide reductase Homo sapiens ?
-
 ?
1.1.1.188 additional information PGFS does not catalyze the reduction of prostaglandin E2 Homo sapiens ?
-
 ?
1.1.1.188 prostaglandin D2 + NADPH + H+
-
 Homo sapiens prostaglandin 9alpha,11beta-F2 + NADP+
-
 ?
1.1.1.188 prostaglandin D2 + NADPH + H+
-
 Homo sapiens 9alpha,11beta-prostaglandin F2alphabeta + NADP+
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Homo sapiens prostaglandin F2alpha + NADP+
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Homo sapiens 9alpha,11alpha-prostaglandin F2alpha + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.188 PGD2 11-ketoreductase
-
 Homo sapiens
1.1.1.188 PGFS
-
 Homo sapiens
1.1.1.188 PGH2 9,11-endoperoxide reductase
-
 Homo sapiens
1.1.1.188 prostaglandin F synthase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.188 30
-
 assay at, PGH2 9,11-endoperoxide reductase activity Homo sapiens
1.1.1.188 37
-
 assay at, PGD2 11-ketoreductase activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 6.5
-
 assay at, PGD2 11-ketoreductase activity and PGH2 9,11-endoperoxide reductase activity Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Homo sapiens