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Literature summary extracted from

  • Garrison, P.N.; Robinson, A.K.; Pekarsky, Y.; Croce, C.M.; Barnes, L.D.
    Phosphorylation of the human Fhit tumor suppressor on tyrosine 114 in Escherichia coli and unexpected steady state kinetics of the phosphorylated forms (2005), Biochemistry, 44, 6286-6292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.29 co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1, expression of nonphosphorylized enzyme in Escherichia coli strain SG100 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.29 additional information
-
 additional information steady state kinetics of the phosphorylated enzyme form Homo sapiens
3.6.1.29 0.00066
-
 P1,P3-bis(5'-adenosyl)triphosphate diphosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens
3.6.1.29 0.00067
-
 P1,P3-bis(5'-adenosyl)triphosphate monophosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens
3.6.1.29 0.0016
-
 P1,P3-bis(5'-adenosyl)triphosphate nonphosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.29 Mg2+ dependent on Homo sapiens
3.6.1.29 Mn2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.29 P1,P3-bis(5'-adenosyl)triphosphate + H2O Homo sapiens
-
 ADP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.29 Homo sapiens P49789
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.29 phosphoprotein Fhit is phosphorylated in vivo, and is phosphorylated in vitro by Src kinase Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.29 co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1 by ion exchange chromatography, gel filtration, and another step of ion exchange chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP the dimeric enzyme contains a catalytic histidine triad with H96 forming a covalent intermediate at the alpha-phosphate of P1,P3-bis(5'-adenosyl)triphosphate, S128, H96 and H98 are essential for the reaction, structure overview Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.29 P1,P3-bis(5'-adenosyl)triphosphate + H2O
-
 Homo sapiens ADP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.29 dimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.29 Fhit
-
 Homo sapiens
3.6.1.29 fragile histidine triad
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.29 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.29 0.02
-
 P1,P3-bis(5'-adenosyl)triphosphate diphosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens
3.6.1.29 0.38
-
 P1,P3-bis(5'-adenosyl)triphosphate monophosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens
3.6.1.29 2.74
-
 P1,P3-bis(5'-adenosyl)triphosphate nonphosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens
3.6.1.29 3 6 P1,P3-bis(5'-adenosyl)triphosphate nonphosphorylated recombinant enzyme, pH 6.8, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.29 6.8
-
 assay at Homo sapiens