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Literature summary extracted from

  • Cervellati, C.; Dallocchio, F.; Bergamini, C.M.; Cook, P.F.
    Role of methionine-13 in the catalytic mechanism of 6-phosphogluconate dehydrogenase from sheep liver (2005), Biochemistry, 44, 2432-2440.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.44 M13C mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
1.1.1.44 M13F mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
1.1.1.44 M13I mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
1.1.1.44 M13Q mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
1.1.1.44 M13V mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 NADPH competitive inhibition versus NADP+ with 6-phospho-D-gluconate equal to its Km and at a saturating concentration Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.002
-
 NADP+ wild type enzyme Ovis aries
1.1.1.44 0.0064
-
 NADP+ mutant enzyme M13C Ovis aries
1.1.1.44 0.019
-
 NADP+ mutant enzyme M13F Ovis aries
1.1.1.44 0.019
-
 NADP+ mutant enzyme M13Q Ovis aries
1.1.1.44 0.026
-
 NADP+ mutant enzyme M13V Ovis aries
1.1.1.44 0.032
-
 NADP+ mutant enzyme M13I Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Ovis aries P00349
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44 Ni-NTA resin column chromatography and 2?5?ADP agarose chromatography Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Ovis aries
1.1.1.44 6PGDH
-
 Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+
-
 Ovis aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.44 0.00065
-
 NADPH mutant enzyme M13C, at a saturating concentration Ovis aries
1.1.1.44 0.001
-
 NADPH mutant enzyme M13I, at a saturating concentration Ovis aries
1.1.1.44 0.0011
-
 NADPH wild type enzyme, at a saturating concentration Ovis aries
1.1.1.44 0.0015
-
 NADPH mutant enzyme M13F, at a saturating concentration Ovis aries
1.1.1.44 0.0021
-
 NADPH mutant enzyme M13Q, at a saturating concentration Ovis aries
1.1.1.44 0.006
-
 NADPH mutant enzyme M13V, at a saturating concentration Ovis aries