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Literature summary extracted from

  • Shenoy, A.R.; Sreenath, N.; Podobnik, M.; Kovacevic, M.; Visweswariah, S.S.
    The Rv0805 gene from Mycobacterium tuberculosis encodes a 3',5'-cyclic nucleotide phosphodiesterase: biochemical and mutational analysis (2005), Biochemistry, 44, 15695-15704.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.17 expression in Escherichia coli and in Mycobacterium leprae Mycobacterium tuberculosis
3.1.4.17 overexpression in Mycobacterium smegmatis and Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.17 homology modeling of enzyme, based on kidny bean PAP and Mre nuclease Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.17 D21A specific activity for cAMP hydrolysis is nearly identical to wild-type value Mycobacterium tuberculosis
3.1.4.17 D21A 90% loss of catalytic activity Mycobacterium tuberculosis
3.1.4.17 D66A specific activity for cAMP hydrolysis is 4fold lower than wild-type value Mycobacterium tuberculosis
3.1.4.17 D66A 75% of wild-type activity, sigmoidal kinetics with respect to Mn2+ Mycobacterium tuberculosis
3.1.4.17 H169A 60% of wild-type activity Mycobacterium tuberculosis
3.1.4.17 H169A specific activity for cAMP hydrolysis is nearly identical to wild-type value Mycobacterium tuberculosis
3.1.4.17 H207A 30% of wild-type activity Mycobacterium tuberculosis
3.1.4.17 H207A specific activity for cAMP hydrolysis is 2.4 fold lower than wild-type value Mycobacterium tuberculosis
3.1.4.17 H23A 60% of wild-type activity Mycobacterium tuberculosis
3.1.4.17 H23A specific activity for cAMP hydrolysis is 1.4fold higher than wild-type value Mycobacterium tuberculosis
3.1.4.17 additional information overexpression in Escherichia coli and in Mycobacterium leprae results in reduced intracellular cAMP levels Mycobacterium tuberculosis
3.1.4.17 N97A almost complete loss of activity Mycobacterium tuberculosis
3.1.4.17 N97A specific activity for cAMP hydrolysis is nearly identical to wild-type value Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.17 EDTA complete loss of activity for mutants H23A, H169A, H207A Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 0.15
-
 3',5'-cAMP
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.17 Mn2+ Km value and Hill constant for wild-type 0.021 mM and 1, respectively, for mutant D21A 0.021 mM and 4.1, respectively, for mutant H23A 0.053 mM and 1, respectively, for mutant D66A, 0.072 and 6.4, respectively, for mutant H169A 0.022 and 1.7, respectively, for mutant H207A, 0.062 and 3.1, respectively Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.17 additional information Mycobacterium tuberculosis Rv0805 protein could have an important role to play in regulating cAMP levels in Mycobacterium tuberculosis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Mycobacterium tuberculosis
-
-
-
3.1.4.17 Mycobacterium tuberculosis
-
 product of Rv0805 gene
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 3',5'-cAMP + H2O
-
 Mycobacterium tuberculosis 5'-AMP
-
 ?
3.1.4.17 3',5'-cGMP + H2O
-
 Mycobacterium tuberculosis 5'-GMP
-
 ?
3.1.4.17 additional information Rv0805 protein could have an important role to play in regulating cAMP levels in Mycobacterium tuberculosis Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.17 Rv0805 protein
-
 Mycobacterium tuberculosis