Literature summary extracted from

Shiryaev, S.A.; Ratnikov, B.I.; Chekanov, A.V.; Sikora, S.; Rozanov, D.V.; Godzik, A.; Wang, J.; Smith, J.W.; Huang, Z.; Lindberg, I.; Samuel, M.A.; Diamond, M.S.; Strongin, A.Y.
Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase (2006), Biochem. J., 393, 503-511.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.21.98	H51A	less than 1% of wild-type activity, no autolytic conversion into mature enzyme	West Nile virus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.21.98	alpha1-anitrypsin	-	West Nile virus
3.4.21.98	Aprotinin	-	West Nile virus
3.4.21.98	deca-D-Arg-NH2	-	West Nile virus
3.4.21.98	dodeca-D-Arg-NH2	-	West Nile virus
3.4.21.98	dodeka-D-Arg-NH2	-	West Nile virus
3.4.21.98	hepta-D-Arg-NH2	-	West Nile virus
3.4.21.98	hexa-D-Arg-NH2	-	West Nile virus
3.4.21.98	nona-D-Arg-NH2	reduces infection in primary neurons	West Nile virus
3.4.21.98	octa-D-Arg-NH2	-	West Nile virus
3.4.21.98	undeca-D-Arg-NH2	-	West Nile virus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.21.98	0.041	-	Boc-RVRR-7-amido-4-methylcoumarin	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.043	-	Pyr-RTKR-7-amido-4-methylcoumarin	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.146	-	Z-RR-7-amido-4-methylcoumarin	pH 8.0, 18°C	West Nile virus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.21.98	30000	-	x * 30000, mature protein, SDS-PAGE	West Nile virus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.98	West Nile virus	-	recombinantenzyme	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.21.98	proteolytic modification	autolytic conversion of 36 kDa NS2B-NS3 construct into the 30 kDa NS3 enzyme	West Nile virus

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.21.98	recombinant protein, autolytic conversion of 36 kDa NS2B-NS3 construct into the 30 kDa NS3 enzyme	West Nile virus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.98	Boc-RVRR-7-amido-4-methylcoumarin + H2O	-	West Nile virus	?	-	?
3.4.21.98	additional information	no substrate: Boc-AGPR-AMC, Boc-QAR-AMC, Boc-O-benzyl-EGR-pNA	West Nile virus	?	-	?
3.4.21.98	myelin basic protein + H2O	-	West Nile virus	?	-	?
3.4.21.98	Pyr-RTKR-7-amido-4-methylcoumarin + H2O	-	West Nile virus	?	-	?
3.4.21.98	Z-RR-7-amido-4-methylcoumarin + H2O	-	West Nile virus	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.21.98	?	x * 30000, mature protein, SDS-PAGE	West Nile virus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4.21.98	0.062	-	Z-RR-7-amido-4-methylcoumarin	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.073	-	Boc-RVRR-7-amido-4-methylcoumarin	pH 8.0, 18°C	West Nile virus
3.4.21.98	43	-	Pyr-RTKR-7-amido-4-methylcoumarin	pH 8.0, 18°C	West Nile virus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.4.21.98	0.000001	-	undeca-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000001	-	dodeka-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000002	-	deca-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000006	-	nona-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000017	-	octa-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000026	-	Aprotinin	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000041	-	hepta-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.000478	-	hexa-D-Arg-NH2	pH 8.0, 18°C	West Nile virus
3.4.21.98	0.00059	-	alpha1-antitrypsin	pH 8.0, 18°C	West Nile virus

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Release 2023.1 (January 2023)