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Literature summary extracted from

  • Han, J.S.; Ishikawa, K.
    Active site of Zn(2+)-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1 (2005), Archaea, 1, 311-317.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.261 D144A lower activity compared to the wild type enzyme Aeropyrum pernix
1.1.1.261 D144N lower activity compared to the wild type enzyme Aeropyrum pernix
1.1.1.261 D191N lower activity compared to the wild type enzyme Aeropyrum pernix
1.1.1.261 D191N/H271A lower activity compared to the wild type enzyme Aeropyrum pernix
1.1.1.261 H271A lower activity compared to the wild type enzyme Aeropyrum pernix
1.1.1.261 H287A lower activity compared to the wild type enzyme Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.261 0.036
-
 NADH mutant enzyme H287A Aeropyrum pernix
1.1.1.261 0.039
-
 NADPH mutant enzyme D144A Aeropyrum pernix
1.1.1.261 0.0477
-
 NADH wild type enzyme Aeropyrum pernix
1.1.1.261 0.05
-
 dihydroxyacetone phosphate wild type enzyme, with NADPH as coenzyme Aeropyrum pernix
1.1.1.261 0.086
-
 NADPH wild type enzyme Aeropyrum pernix
1.1.1.261 0.165
-
 NADPH mutant enzyme D144N Aeropyrum pernix
1.1.1.261 0.177
-
 dihydroxyacetone phosphate wild type enzyme, with NADH as coenzyme Aeropyrum pernix
1.1.1.261 0.213
-
 NADH mutant enzyme D144A Aeropyrum pernix
1.1.1.261 0.218
-
 dihydroxyacetone phosphate mutant enzyme D144A, with NADPH as coenzyme Aeropyrum pernix
1.1.1.261 0.259
-
 dihydroxyacetone phosphate mutant enzyme H287A, with NADH as coenzyme Aeropyrum pernix
1.1.1.261 0.296
-
 NADH mutant enzyme D191N Aeropyrum pernix
1.1.1.261 0.321
-
 NADH mutant enzyme H271A Aeropyrum pernix
1.1.1.261 0.36
-
 dihydroxyacetone phosphate mutant enzyme D144N, with NADPH as coenzyme Aeropyrum pernix
1.1.1.261 0.415
-
 NADH mutant enzyme D144N Aeropyrum pernix
1.1.1.261 0.633
-
 dihydroxyacetone phosphate mutant enzyme D191N, with NADH as coenzyme Aeropyrum pernix
1.1.1.261 1.09
-
 dihydroxyacetone phosphate mutant enzyme H271A, with NADH as coenzyme Aeropyrum pernix
1.1.1.261 1.12
-
 dihydroxyacetone phosphate mutant enzyme D144A, with NADH as coenzyme Aeropyrum pernix
1.1.1.261 1.12
-
 dihydroxyacetone phosphate mutant enzyme D144N, with NADH as coenzyme Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.261 Zn2+ the specific activity abruptly increases until 0.5 mM ZnCl2 is added, and it is suppressed in the wild type when the ZnCl2 concentration exceeds 1.0 mM Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.261 Aeropyrum pernix
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.261 4°C, 50 mM Tris-HCl buffer (pH 8.0) containing ZnCl2, 1 h, no loss of activity Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.261 dihydroxyacetone phosphate + NAD(P)H
-
 Aeropyrum pernix sn-glycerol-1-phosphate + NAD(P)+
-
 r
1.1.1.261 sn-glycerol-1-phosphate + NAD+
-
 Aeropyrum pernix dihydroxyacetone phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.261 monomer
-
 Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
1.1.1.261 Gro1PDH
-
 Aeropyrum pernix
1.1.1.261 Zn2+-dependent sn-glycerol-1-phosphate dehydrogenase
-
 Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.261 96
-
-
 Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.261 0.493
-
 dihydroxyacetone phosphate mutant enzyme H271A Aeropyrum pernix
1.1.1.261 1.1
-
 dihydroxyacetone phosphate mutant enzyme D144A Aeropyrum pernix
1.1.1.261 2.945
-
 dihydroxyacetone phosphate mutant enzyme H287A Aeropyrum pernix
1.1.1.261 3 6 dihydroxyacetone phosphate mutant enzyme H287A Aeropyrum pernix
1.1.1.261 3.27
-
 dihydroxyacetone phosphate mutant enzyme D191N Aeropyrum pernix
1.1.1.261 3.3
-
 dihydroxyacetone phosphate wild type enzyme Aeropyrum pernix
1.1.1.261 4.998
-
 dihydroxyacetone phosphate wild type enzyme Aeropyrum pernix
1.1.1.261 8
-
 dihydroxyacetone phosphate mutant enzyme D144N Aeropyrum pernix
1.1.1.261 18.77
-
 dihydroxyacetone phosphate mutant enzyme D144A Aeropyrum pernix
1.1.1.261 60.47
-
 dihydroxyacetone phosphate mutant enzyme D144N Aeropyrum pernix

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.261 NAD(P)H
-
 Aeropyrum pernix