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Literature summary extracted from

  • Yaoi, K.; Nakai, T.; Kameda, Y.; Hiyoshi, A.; Mitsuishi, Y.
    Cloning and characterization of two xyloglucanases from Paenibacillus sp. strain KM21 (2005), Appl. Environ. Microbiol., 71, 7670-7678.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 expression in Escherichia coli Paenibacillus sp.
3.2.1.155 expression in Escherichia coli Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information KM-value for xyloglucan is 1.2 mg/ml Paenibacillus sp.
3.2.1.151 additional information
-
 additional information KM-value for xyloglucan is 2.0 mg/ml Paenibacillus sp.
3.2.1.155 additional information
-
 additional information KM-value for xyloglucan is 1.2 mg/ml Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 40000
-
 x * 40000, SDS-PAGE Paenibacillus sp.
3.2.1.151 105000
-
 x * 105000, SDS-PAGE Paenibacillus sp.
3.2.1.155 105000
-
 x * 105000, SDS-PAGE Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Paenibacillus sp. Q3MUH7
-
-
3.2.1.151 Paenibacillus sp. Q3MUH8
-
-
3.2.1.151 Paenibacillus sp. KM21 Q3MUH7
-
-
3.2.1.151 Paenibacillus sp. KM21 Q3MUH8
-
-
3.2.1.155 Paenibacillus sp. Q3MUH7
-
-
3.2.1.155 Paenibacillus sp. KM21 Q3MUH7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.151 recombinant Paenibacillus sp.
3.2.1.155 recombinant Paenibacillus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.151 additional information
-
-
 Paenibacillus sp.
3.2.1.155 additional information
-
-
 Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 additional information no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan Paenibacillus sp. ?
-
 ?
3.2.1.151 additional information no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan Paenibacillus sp. KM21 ?
-
 ?
3.2.1.151 XXXGXXXG + H2O
-
 Paenibacillus sp. XXX + XXXG + GXXXG
-
 ?
3.2.1.151 XXXGXXXG + H2O
-
 Paenibacillus sp. KM21 XXX + XXXG + GXXXG
-
 ?
3.2.1.151 XXXGXXXG + H2O
-
 Paenibacillus sp. 2 XXXG
-
 ?
3.2.1.151 XXXGXXXG + H2O
-
 Paenibacillus sp. KM21 2 XXXG
-
 ?
3.2.1.151 xyloglucan + H2O XEG5 randomly cleaves the xyloglucan main chain Paenibacillus sp. xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O XEG74 has dual endo-mode and exo-mode activities Paenibacillus sp. xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O XEG74 has dual endo-mode and exo-mode activities Paenibacillus sp. KM21 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O XEG5 randomly cleaves the xyloglucan main chain Paenibacillus sp. KM21 xyloglucan oligosaccharides
-
 ?
3.2.1.155 additional information no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan Paenibacillus sp. ?
-
 ?
3.2.1.155 additional information no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan Paenibacillus sp. KM21 ?
-
 ?
3.2.1.155 XXXGXXXG + H2O
-
 Paenibacillus sp. XXX + XXXG + GXXXG
-
 ?
3.2.1.155 xyloglucan + H2O XEG74 has dual endo-mode and exo-mode activities Paenibacillus sp. xyloglucan oligosaccharides
-
 ?
3.2.1.155 xyloglucan + H2O XEG74 has dual endo-mode and exo-mode activities Paenibacillus sp. KM21 xyloglucan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 105000, SDS-PAGE Paenibacillus sp.
3.2.1.151 ? x * 40000, SDS-PAGE Paenibacillus sp.
3.2.1.155 ? x * 105000, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 XEG5
-
 Paenibacillus sp.
3.2.1.151 XEG74
-
 Paenibacillus sp.
3.2.1.151 xyloglucan-specific endo-beta-1,4-glucanase
-
 Paenibacillus sp.
3.2.1.155 XEG74
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 50 55
-
 Paenibacillus sp.
3.2.1.151 60 70
-
 Paenibacillus sp.
3.2.1.155 60 70
-
 Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 55
-
 stable up to Paenibacillus sp.
3.2.1.151 55
-
 10 min, less than 10% loss of activity Paenibacillus sp.
3.2.1.155 55
-
 stable up to Paenibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 3
-
 xyloglucan
-
 Paenibacillus sp.
3.2.1.151 12.7
-
 xyloglucan
-
 Paenibacillus sp.
3.2.1.151 77.6
-
 xyloglucan
-
 Paenibacillus sp.
3.2.1.155 3
-
 xyloglucan
-
 Paenibacillus sp.
3.2.1.155 77.6
-
 xyloglucan
-
 Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5.5 6.5
-
 Paenibacillus sp.
3.2.1.151 6 6.5
-
 Paenibacillus sp.
3.2.1.155 6 6.5
-
 Paenibacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.151 5 7.5 stable Paenibacillus sp.
3.2.1.151 5 8 at 45°C, stable Paenibacillus sp.
3.2.1.155 5 7.5 stable Paenibacillus sp.