Literature summary extracted from

Liu, B.; Li, X.; Gao, R.; Zhou, W.; Xie, G.; Bartlam, M.; Pang, H.; Feng, Y.; Rao, Z.
Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 (2004), Acta Crystallogr. Sect. D, 60, 577-579.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.6.1.1	expression in Escherichia coli	Pyrococcus horikoshii

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.6.1.1	purified recombinant enzyme, hanging drop vapour diffusion method, 20 mg/ml protein in 5 mM Tris-HCl, pH 8.0, and 50 mM NaCl, 18°C, 0.001 ml versus 0.001 ml reservoir solution containing 8% PEG 4000, 0.1 M sodium acetate, pH 4.5, for needle-shaped crystals, and 3.8% PEG 4000, 0.1 M sodium acetate, pH 5.0-5.2, for large crystals, X-ray diffraction structure determination and analysis at 2.7 A resolution	Pyrococcus horikoshii

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.6.1.1	Pyrococcus horikoshii	-	strain OT3	-
3.6.1.1	Pyrococcus horikoshii OT-3	-	strain OT3	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.6.1.1	recombinant enzyme from Escherichia coli	Pyrococcus horikoshii

Synonyms

EC Number	Synonyms	Comment	Organism
3.6.1.1	inorganic pyrophosphatase	-	Pyrococcus horikoshii
3.6.1.1	PPase	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)