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Literature summary extracted from

  • Tete-Favier, F.; Cobessi, D.; Leonard, G.A.; Azza, S.; Talfournier, F.; Boschi-Muller, S.; Branlant, G.; Aubry, A.
    Crystallization and preliminary X-ray diffraction studies of the peptide methionine sulfoxide reductase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 1194-1197.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 gene msrA, expression in strain B834(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.11 15-50 mg/ml purified recombinant MsrA in 50 mM Tris-HCl, pH 8.0, 2 mM EDTA, and 10 mM DTT, hanging drop vapour diffusion method, droplet size is 0.004-0.008 ml, equal volumes of protein and precipitant solution, X-ray diffraction structure determination and analysis at 1.9 A resolution Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin Escherichia coli MsrA is involved in the antioxidant defense peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11 recombinant MsrA from strain B834(DE3) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 peptide-L-methionine-(S)-S-oxide + DTT
-
 Escherichia coli peptide-L-methionine + DTT disulfide + H2O
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin
-
 Escherichia coli peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin MsrA is involved in the antioxidant defense Escherichia coli peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 MsrA
-
 Escherichia coli
1.8.4.11 peptide methionine sulfoxide reductase
-
 Escherichia coli
1.8.4.11 PMSR
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 dithiothreitol
-
 Escherichia coli
1.8.4.11 thioredoxin
-
 Escherichia coli