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Literature summary extracted from

  • Janer, C.; Rohr, L.M.; Pelaez, C.; Laloi, M.; Cleusix, V.; Requena, T.; Meile, L.
    Hydrolysis of oligofructoses by the recombinant beta-fructofuranosidase from Bifidobacterium lactis (2004), Syst. Appl. Microbiol., 27, 279-285.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 bovine serum albumin 41% activation at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 DTT 26% activation at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 EDTA 32% activation at 1 mM Bifidobacterium animalis subsp. lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 gene cscA, genetic organization, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue and as soluble, His-tagged enzyme in strain BL21(DE3) Bifidobacterium animalis subsp. lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 4-hydroxymercuribenzoate complete inhibition at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 Ca2+ 15% inhibition at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 Co2+ 38% inhibition at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 Cu2+ complete inhibition at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 Fe2+ 55% inhibition at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 SDS complete inhibition at 1 mM Bifidobacterium animalis subsp. lactis
3.2.1.26 Zn2+ 56% inhibition at 1 mM Bifidobacterium animalis subsp. lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 cytoplasm
-
 Bifidobacterium animalis subsp. lactis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.26 additional information the enzyme is not affected by 1 mM Mn2+ Bifidobacterium animalis subsp. lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 60000
-
 x * 60000, recombinant His-tagged enzyme, SDS-PAGE Bifidobacterium animalis subsp. lactis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Bifidobacterium animalis subsp. lactis Q564C7 gene cscA, strain DSM 10140
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 recombinant soluble, His-tagged CscA 238fold from Escherichia coli strain BL21(DE3) by metal affinity chromatography Bifidobacterium animalis subsp. lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 additional information
-
-
 Bifidobacterium animalis subsp. lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 levan + H2O low activity Bifidobacterium animalis subsp. lactis D-fructose + ?
-
 ?
3.2.1.26 additional information no activity with melezitose, raffinose is a poor substrate Bifidobacterium animalis subsp. lactis ?
-
 ?
3.2.1.26 Raftiline HP + H2O low activity Bifidobacterium animalis subsp. lactis D-glucose + D-fructose
-
 ?
3.2.1.26 raftiline LS + H2O
-
 Bifidobacterium animalis subsp. lactis D-glucose + D-fructose
-
 ?
3.2.1.26 raftilose + H2O best substrate for CscA Bifidobacterium animalis subsp. lactis D-fructose
-
 ?
3.2.1.26 sucrose + H2O low activity Bifidobacterium animalis subsp. lactis D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? x * 60000, recombinant His-tagged enzyme, SDS-PAGE Bifidobacterium animalis subsp. lactis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 beta-(1-2)-fructofuranosidase
-
 Bifidobacterium animalis subsp. lactis
3.2.1.26 CscA
-
 Bifidobacterium animalis subsp. lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 37
-
 assay at Bifidobacterium animalis subsp. lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6
-
 assay at Bifidobacterium animalis subsp. lactis