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Literature summary extracted from

  • An, C.L.; Lim, W.J.; Hong, S.Y.; Shin, E.C.; Kim, M.K.; Lee, J.R.; Park, S.R.; Woo, J.G.; Lim, Y.P.; Yun, H.D.
    Structural and biochemical analysis of the asc operon encoding 6-phospho-beta-glucosidase in Pectobacterium carotovorum subsp. carotovorum LY34 (2005), Res. Microbiol., 156, 145-153.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.86
-
 Pectobacterium carotovorum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.86 E182A inactive mutant Pectobacterium carotovorum
3.2.1.86 E374A inactive mutant Pectobacterium carotovorum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.86 Co2+ 5 mM, 12% inhibition Pectobacterium carotovorum
3.2.1.86 Cu2+ 5 mM, 8% inhibition Pectobacterium carotovorum
3.2.1.86 Hg2+ 5 mM, complete inhibition Pectobacterium carotovorum
3.2.1.86 Mn2+ 5 mM, 14% inhibition Pectobacterium carotovorum
3.2.1.86 Zn2+ 5 mM, 13% inhibition Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.21
-
 p-nitrophenyl-beta-D-glucopyranoside pH 7.0, 40°C Pectobacterium carotovorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.86 Ca2+ enhances activity, at 5 mM 11% increase in activity compared to activity without added ion Pectobacterium carotovorum
3.2.1.86 Mg2+ enhances activity, maximal hydrolysis of p-nitrophenyl-beta-D-glucopyranoside at 5 mM, 7% increase in activity compared to activity without added ion Pectobacterium carotovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.86 53000
-
 x * 53000, SDS-PAGE Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Pectobacterium carotovorum
-
 subsp. carotovorum LY34
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.86
-
 Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.86 565
-
-
 Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 4-methylumbelliferyl-beta-D-glucoside + H2O
-
 Pectobacterium carotovorum 4-methylumbelliferone + D-glucose
-
 ?
3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Pectobacterium carotovorum p-nitrophenol + D-glucose
-
 ?
3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
-
 Pectobacterium carotovorum p-nitrophenol + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.86 ? x * 53000, SDS-PAGE Pectobacterium carotovorum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.86 AscB protein
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.86 40
-
 pH 7.0, hydrolysis of p-nitrophenyl-beta-D-glucopyranoside Pectobacterium carotovorum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.86 20 50 20°C: 55% of maximal activity, 50°C: 48% of maximal activity Pectobacterium carotovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.86 60
-
 10 min, 39% loss of activity Pectobacterium carotovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.86 7
-
-
 Pectobacterium carotovorum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.86 4 9 pH 4.0: about 60% of maximal activity, pH 9.0: about 30% of maximal activity Pectobacterium carotovorum