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Literature summary extracted from

  • Golich, F.C.; Han, M.; Crowder, M.W.
    Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli (2006), Protein Expr. Purif., 47, 634-639.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 gene pepN, cloning from genomic DNA of strain W3110, overexpression in strain BL21(DE3), method optimization Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 additional information poor or no inhibition by Fe2+ Escherichia coli
3.4.11.2 Zn2+ recombinant enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 additional information
-
 additional information steady-state kinetics Escherichia coli
3.4.11.2 0.2
-
 L-Leu-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
3.4.11.2 0.28
-
 L-Arg-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
3.4.11.2 0.38
-
 L-Ala-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Fe2+ 5 mol per mol of enzyme Escherichia coli
3.4.11.2 Zn2+ 0.1 mol per mole of enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 98750
-
 mass spectrometry Escherichia coli
3.4.11.2 99000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Escherichia coli
-
 gene pepN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 recombinant enzyme 3.8fold from overexpressing strain BL21(DE3) by anion exchange chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.2 964
-
 purified recombinant enzyme, substrate L-Ala-4-nitroanilide Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Escherichia coli L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Arg-4-nitroanilide + H2O
-
 Escherichia coli L-Arg + 4-nitroaniline
-
 ?
3.4.11.2 L-Leu-4-nitroanilide + H2O
-
 Escherichia coli L-Leu + 4-nitroaniline
-
 ?
3.4.11.2 additional information no activity with L-Val-4-nitroanilide, L-Met-4-nitroanilide, and L-Pro-4-nitroanilide Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer 1 * 98750, recombinant enzyme, mass spectrometry Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase N
-
 Escherichia coli
3.4.11.2 PepN
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.2 4.4
-
 L-Leu-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
3.4.11.2 158
-
 L-Arg-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
3.4.11.2 354
-
 L-Ala-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7
-
 assay at Escherichia coli