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Literature summary extracted from

  • Bageshwar, U.K.; Premkumar, L.; Gokhman, I.; Savchenko, T.; Sussman, J.L.; Zamir, A.
    Natural protein engineering: a uniquely salt-tolerant, but not halophilic, alpha-type carbonic anhydrase from algae proliferating in low- to hyper-saline environments (2004), Protein Eng. Des. Sel., 17, 191-200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expression in Escherichia coli Dunaliella salina

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide IC50: 97 nM Dunaliella salina
4.2.1.1 ClO4- IC50: 2.2 mM Dunaliella salina
4.2.1.1 I- IC50: 100 mM Dunaliella salina
4.2.1.1 N3- IC50: about 50 mM Dunaliella salina
4.2.1.1 p-aminomethylbenzenesulfonamide IC50: 0.0058 mM Dunaliella salina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 37.1
-
 H2CO3 pH 6.8, 0.1 M NaCl Dunaliella salina
4.2.1.1 37.3
-
 H2CO3 pH 6.8, in absence of NaCl Dunaliella salina
4.2.1.1 45
-
 H2CO3 pH 6.8, 0.5 M NaCl Dunaliella salina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.1 cell surface
-
 Dunaliella salina 9986
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 KCl enzyme retains activity at salt concentrations ranging from low salt to at least 1.5 M NaCl or KCl for CO2 hydration, 2.0 M NaCl for esterase activity and 0.5 M for bicarbonate dehydration Dunaliella salina
4.2.1.1 NaCl enzyme retains activity at salt concentrations ranging from low salt to at least 1.5 M NaCl or KCl for CO2 hydration, 2.0 M NaCl for esterase activity and 0.5 M for bicarbonate dehydration Dunaliella salina
4.2.1.1 Zn Zn metalloenzyme Dunaliella salina

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Dunaliella salina P54212
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Dunaliella salina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Dunaliella salina CO2 + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 alpha-type carbonic anhydrase
-
 Dunaliella salina
4.2.1.1 dCA I
-
 Dunaliella salina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 39400
-
 H2CO3 pH 6.8, in absence of NaCl Dunaliella salina
4.2.1.1 41600
-
 H2CO3 pH 6.8, 0.1 M NaCl Dunaliella salina
4.2.1.1 45000
-
 H2CO3 pH 6.8, 0.5 M NaCl Dunaliella salina

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.000097
-
 IC50: 97 nM Dunaliella salina acetazolamide
4.2.1.1 0.0058
-
 IC50: 0.0058 mM Dunaliella salina p-aminomethylbenzenesulfonamide
4.2.1.1 2.2
-
 IC50: 2.2 mM Dunaliella salina ClO4-
4.2.1.1 50
-
 IC50: about 50 mM Dunaliella salina N3-
4.2.1.1 100
-
 IC50: 100 mM Dunaliella salina I-