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Literature summary extracted from

  • Ng, I.S.; Tsai, S.W.
    Investigation of lipases from various Carica papaya varieties for hydrolysis of olive oil and kinetic resolution of (R,S)-profen 2,2,2-trifluoroethyl thioesters (2006), Process Biochem., 41, 540-546.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information detailed kinetic analysis and thermodynamics of different isozymes, overview Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Carica papaya
-
 diverse varieties from different geographic locations, e.g. Indo, China, and Taiwan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 four isozymes, partially Carica papaya

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 9.1
-
 partially purified isozyme CPL-Taiwan, substrate olive oil Carica papaya
3.1.1.3 12
-
 partially purified isozyme CPL-China, substrate olive oil Carica papaya
3.1.1.3 26.7
-
 partially purified isozyme CPL, substrate olive oil Carica papaya
3.1.1.3 40.9
-
 partially purified isozyme CPL-Indo, substrate olive oil Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 (R,S)-naproxen 2,2,2-trifluoroethyl thioester (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya (S)-naproxen + 2,2,2-trifluorothioethanol + (R)-naproxen 2,2,2-trifluoroethyl thioester
-
 ?
3.1.1.3 fenoprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ?
-
 ?
3.1.1.3 flurbiprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ?
-
 ?
3.1.1.3 ibuprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ?
-
 ?
3.1.1.3 ketoprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ?
-
 ?
3.1.1.3 additional information temperature-dependent substrate specificity of isozymes with (R,S)-profen 2,2,2-trifluoroethyl thioesters, overview Carica papaya ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Carica papaya dibutyrin + butyrate
-
 ?
3.1.1.3 triolein + H2O olive oil in aqueous solution, enantioselective hydrolysis Carica papaya diolein + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 CPL
-
 Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 additional information
-
 temperature-dependent substrate specificity of isozymes with (R,S)-profen 2,2,2-trifluoroethyl thioesters, overview Carica papaya
3.1.1.3 40
-
 assay at Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
-
 Carica papaya