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Literature summary extracted from

  • Ungewickell, A.; Hugge, C.; Kisseleva, M.; Chang, S.C.; Zou, J.; Feng, Y.; Galyov, E.E.; Wilson, M.; Majerus, P.W.
    The identification and characterization of two phosphatidylinositol-4,5-bisphosphate 4-phosphatases (2005), Proc. Natl. Acad. Sci. USA, 102, 18854-18859.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.78 type I isozyme, DNA and amino acid sequence determination and analysis, transient transfection of functional enzyme to HeLa and COS-7 cells leading to increased EGFR degradation via increased phosphatidylinositol-5-phosphate content, functional overexpression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus infection system, stable inducible expression of labeled isozyme I in HEK293 cells Homo sapiens
3.1.3.78 type II isozyme, DNA and amino acid sequence determination and analysis, transient transfection of functional enzyme to HeLa and COS-7 cells leading to increased EGFR degradation via increased phosphatidylinositol-5-phosphate content, functional overexpression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.78 additional information expression of isozyme I siRNA in HeLa cells does not affect phosphatidylinositol-4,5-bisphosphate levels or EGFR degradation Homo sapiens
3.1.3.78 additional information expression of isozyme II siRNA in HeLa cells does not affect phosphatidylinositol-4,5-bisphosphate levels or EGFR degradation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.78 endosome membrane late Homo sapiens 10008
-
3.1.3.78 lysosomal membrane late Homo sapiens 5765
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.78 additional information Homo sapiens the enzyme affects epithelial growth factor receptor EGFR degradation upon epithelial growth factor EGF stimulation via phosphatidylinositol-5-phosphate, overview ?
-
 ?
3.1.3.78 phosphatidylinositol-4,5-bisphosphate + H2O Homo sapiens
-
 phosphatidylinositol-5-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.78 Homo sapiens Q86T03 two isozyme I
-
3.1.3.78 Homo sapiens Q8N4L2 isozyme II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.78 FLAG-tagged isozyme I from Sf9 insect cells by anti-FLAG immunoaffinity chromatography Homo sapiens
3.1.3.78 FLAG-tagged isozyme II from Sf9 insect cells by anti-FLAG immunoaffinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.78 adrenal gland
-
 Homo sapiens
-
3.1.3.78 brain
-
 Homo sapiens
-
3.1.3.78 liver
-
 Homo sapiens
-
3.1.3.78 additional information isozyme II is ubiquitously expressed, comparison of expression patterns of isozymes I and II, overview Homo sapiens
-
3.1.3.78 salivary gland
-
 Homo sapiens
-
3.1.3.78 spleen
-
 Homo sapiens
-
3.1.3.78 thymus
-
 Homo sapiens
-
3.1.3.78 thyroid gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.78 additional information the enzyme affects epithelial growth factor receptor EGFR degradation upon epithelial growth factor EGF stimulation via phosphatidylinositol-5-phosphate, overview Homo sapiens ?
-
 ?
3.1.3.78 phosphatidylinositol-4,5-bisphosphate + H2O
-
 Homo sapiens phosphatidylinositol-5-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.78 phosphatidylinositol-4,5-bisphosphate 4-phosphatase I
-
 Homo sapiens
3.1.3.78 phosphatidylinositol-4,5-bisphosphate 4-phosphatase II
-
 Homo sapiens
3.1.3.78 type I PtdIns-4,5-P2 4-Ptase
-
 Homo sapiens
3.1.3.78 type II PtdIns-4,5-P2 4-Ptase
-
 Homo sapiens