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Literature summary extracted from

  • Sandrim, V.C.; Rizzatti, A.C.; Terenzi, H.F.; Jorge, J.A.; Milagres, A.M.; Polizeli, M.L.
    Purification and biochemical characterization of two xylanases produced by Aspergillus caespitosus and their potential for kraft pulp bleaching (2005), Proc. Biochem., 40, 1823-1828.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol isoform xyl I, 59% activation, xyl II, 102% activation Aspergillus caespitosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ba2+ 1 mM, isoform xyl I, 49% inhibition, xyl II, 11% inhibition Aspergillus caespitosus
3.2.1.8 Ca2+ 1 mM, isoform xyl I, 71% inhibition, xyl II, 9% inhibition Aspergillus caespitosus
3.2.1.8 CuSO4 1 mM, isoform xyl I, 85% inhibition, xyl II, 24% inhibition Aspergillus caespitosus
3.2.1.8 Hg2+ both isoform xyl I, xyl II, complete inhibition Aspergillus caespitosus
3.2.1.8 NaCl 1 mM, isoform xyl I, 28% inhibition, xyl II, 19% inhibition Aspergillus caespitosus
3.2.1.8 Zn2+ 1 mM, isoform xyl I, 70% inhibition, xyl II, 10% inhibition Aspergillus caespitosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 7700
-
 isoform xyl II, gelfiltration Aspergillus caespitosus
3.2.1.8 17700
-
 1 * 27000, isoform xyl I, 1 * 17700, isoform xylII, SDS-PAGE Aspergillus caespitosus
3.2.1.8 26300
-
 isoform xyl I, gelfiltration Aspergillus caespitosus
3.2.1.8 27000
-
 1 * 27000, isoform xyl I, 1 * 17700, isoform xylII, SDS-PAGE Aspergillus caespitosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus caespitosus
-
 isoforms xyl I, xyl II, growth in sugar cane bagasse
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 kraft pulp reduction of kappa number and increase in viscosity Aspergillus caespitosus ?
-
 ?
3.2.1.8 xylan + H2O birchwood xylan preferred Aspergillus caespitosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 27000, isoform xyl I, 1 * 17700, isoform xylII, SDS-PAGE Aspergillus caespitosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50 55 both isoforms xyl I, xyl II Aspergillus caespitosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 55
-
 isoform xyl I, half-life 27.3 min, xyl II, half-life above 90 min Aspergillus caespitosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.5 6.5 isoform xyl II Aspergillus caespitosus
3.2.1.8 6.5 7 isoform xyl I Aspergillus caespitosus