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Literature summary extracted from

  • Tejera Garcia, N.A.; Olivera, M.; Iribarne, C.; Lluch, C.
    Partial purification and characterization of a non-specific acid phosphatase in leaves and root nodules of Phaseolus vulgaris (2004), Plant Physiol. Biochem., 42, 585-591.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 additional information phosphate starvation induces the enzyme Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Al3+ strong inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 Co2+ inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 Cu2+ strong inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 Hg2+ strong inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 K+ slight inhibition of root nodule isozymes Phaseolus vulgaris
3.1.3.2 Li+ inhibits isozyme alpha from leaf Phaseolus vulgaris
3.1.3.2 Mn2+ inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 molybdate strong inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 NH4+ slight inhibition of root nodule isozymes Phaseolus vulgaris
3.1.3.2 Pb2+ strong inhibition of leaf and root nodule isozymes Phaseolus vulgaris
3.1.3.2 Zn2+ strong inhibition of leaf and root nodule isozymes Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 soluble
-
 Phaseolus vulgaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 additional information leaf and root nodule isozymes are not affected by Mg2+ and Ca2+, the leaf enzyme is also not affected by NH4+, while the root nodule enzyme is not affected by Li+ Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 34000
-
 2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE Phaseolus vulgaris
3.1.3.2 41000
-
 2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE Phaseolus vulgaris
3.1.3.2 49000
-
 1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE Phaseolus vulgaris
3.1.3.2 51000
-
 1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE Phaseolus vulgaris
3.1.3.2 72000
-
 1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE Phaseolus vulgaris
3.1.3.2 110000
-
 isozyme beta of root nodule, gel filtration Phaseolus vulgaris
3.1.3.2 116000
-
 leaf isozyme alpha, gel filtration Phaseolus vulgaris
3.1.3.2 123000
-
 isozyme gamma of root nodule, gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Phaseolus vulgaris
-
 var. Contender, three isozymes alpha, beta, and gamma
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 120.7fold from root nodules and leaves by ammonium sulfate fractionation, DEAE ion exchange chromatography, ultrafiltration, and gel filtration Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 leaf single isozyme alpha Phaseolus vulgaris
-
3.1.3.2 root nodule two isozymes beta and gamma Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 106.3
-
 purified enzyme Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O high activity Phaseolus vulgaris 4-nitrophenol + phosphate
-
 ?
3.1.3.2 alpha-naphthyl phosphate + H2O high activity Phaseolus vulgaris alpha-naphthol + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Phaseolus vulgaris ADP + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O
-
 Phaseolus vulgaris D-glucose + phosphate
-
 ?
3.1.3.2 FMN + H2O
-
 Phaseolus vulgaris ? + phosphate
-
 ?
3.1.3.2 additional information substrate specificity of leaf and root nodule enzymes, overview Phaseolus vulgaris ?
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O
-
 Phaseolus vulgaris pyruvate + phosphate
-
 ?
3.1.3.2 ribulose 1,5-bisphosphate + H2O high activity Phaseolus vulgaris ? + phosphate
-
 ?
3.1.3.2 uridine 5'-phosphate + H2O
-
 Phaseolus vulgaris uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE Phaseolus vulgaris
3.1.3.2 trimer 2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE Phaseolus vulgaris

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 ACP
-
 Phaseolus vulgaris
3.1.3.2 non-specific acid phosphatase
-
 Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 30
-
-
 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.6
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4 7 inactive below pH 4.0 and above pH 7.0 Phaseolus vulgaris