EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.145 | - |
Raphanus sativus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.145 | Hg2+ | 1 mM, almost complete inhibition | Raphanus sativus | |
3.2.1.145 | iodoacetic acid | 1 mM, about 80% inhibition | Raphanus sativus | |
3.2.1.145 | additional information | not inhibitory: Mn2+, Fe3+, Cu2+, Mg2+, Ba2+, Ca2+, Co2+, Zn2+, Cd2+ | Raphanus sativus | |
3.2.1.145 | sodium dodecylsulfate | 1 mM, about 80% inhibition | Raphanus sativus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.145 | 0.3 | - |
p-nitrophenyl-beta-galactoside | pH 4.0, 37°C | Raphanus sativus | |
3.2.1.145 | 0.77 | - |
beta-(1-3)-D-galactotriose | pH 4.0, 37°C | Raphanus sativus | |
3.2.1.145 | 1.77 | - |
beta-(1-3)-galactobiose | pH 4.0, 37°C | Raphanus sativus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.145 | 33654 | - |
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated | Raphanus sativus |
3.2.1.145 | 44981 | - |
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated | Raphanus sativus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.145 | Raphanus sativus | Q6L619 | recombinant enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.145 | recombinant enzyme expressed in Pichia pastoris | Raphanus sativus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.145 | 64.9 | - |
pH 4.0, 37°C | Raphanus sativus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.145 | beta-(1-3)-D-galactan + H2O | 8% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-(1-3)-D-galactan + D-galactose | - |
? | |
3.2.1.145 | beta-(1-3)-D-galactotriose + H2O | 44% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-(1-3)-D-galactobiose + D-galactose | - |
? | |
3.2.1.145 | beta-(1-3)-galactobiose + H2O | 32% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | D-galactose | - |
? | |
3.2.1.145 | beta-(1-6)-galactopentaose + H2O | 50% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-(1-6)-D-galactotetraose + D-galactose | - |
? | |
3.2.1.145 | beta-(1-6)-galactotetraose + H2O | 68% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-(1-6)-D-galactotriose + D-galactose | - |
? | |
3.2.1.145 | beta-(1-6)-galactotriose + H2O | 49% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-(1-6)-D-galactobiose + D-galactose | - |
? | |
3.2.1.145 | beta-galactosido-(1-3)-beta-galactosido-(1-6)-galactoside + H2O | 42% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-galactosido-(1-3)-beta-galactoside + D-galactose | - |
? | |
3.2.1.145 | beta-galactosido-(1-6)-beta-galactosido-(1-3)-galactoside + H2O | 40% of the activity with p-nitrophenyl-beta-galactoside | Raphanus sativus | beta-galactosido-(1-6)-beta-galactoside + D-galactose | - |
? | |
3.2.1.145 | additional information | specific hydrolysis of beta-1,3 and beta-1,6 galactooligosaccharides | Raphanus sativus | ? | - |
? | |
3.2.1.145 | p-nitrophenyl-beta-galactoside + H2O | - |
Raphanus sativus | p-nitrophenol + beta-galactose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.145 | ? | x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated | Raphanus sativus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.145 | RsBGAL1 | - |
Raphanus sativus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.145 | 50 | - |
- |
Raphanus sativus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.145 | 65 | - |
loss of 90% of activity | Raphanus sativus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.145 | 28.6 | - |
beta-(1-3)-galactobiose | pH 4.0, 37°C | Raphanus sativus | |
3.2.1.145 | 32.5 | - |
beta-(1-3)-D-galactotriose | pH 4.0, 37°C | Raphanus sativus | |
3.2.1.145 | 123 | - |
p-nitrophenyl-beta-galactoside | pH 4.0, 37°C | Raphanus sativus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.145 | 3.5 | 4 | - |
Raphanus sativus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.145 | 2 | 6 | almost inactive below and above | Raphanus sativus |