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Literature summary extracted from

  • Mizutani, M.; Nakanishi, H.; Ema, J.; Ma, S.J.; Noguchi, E.; Inohara-Ochiai, M.; Fukuchi-Mizutani, M.; Nakao, M.; Sakata, K.
    Cloning of beta-primeverosidase from tea leaves, a key enzyme in tea aroma formation (2002), Plant Physiol., 130, 2164-2176.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.149 nutrition beta-primeverosidase is a key enzyme in tea aroma formation Camellia sinensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.149 expression in Escherichia coli Camellia sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.149 61000
-
 x * 61000, SDS-PAGE Camellia sinensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.149 Camellia sinensis Q7X9A9 var sinensis cv Yabukita
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.149
-
 Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.149 leaf
-
 Camellia sinensis
-
3.2.1.149 stem
-
 Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.149 1.7
-
 purified enzyme Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.149 2-phenylethyl beta-primeveroside + H2O pH 6.0, 37°C, 100% activity Camellia sinensis primeverose + 2-phenylethanol
-
 ?
3.2.1.149 additional information no activity with p-nitrophenol beta-D-xyloside and 2-phenylethyl beta-D-glucoside Camellia sinensis ?
-
 ?
3.2.1.149 p-nitrophenyl beta-D-glucoside + H2O pH 6.0, 37°C, 0.5% activity (purified from tea), 0.3% activity (recombinant enzyme) Camellia sinensis p-nitrophenol + D-glucose
-
 ?
3.2.1.149 p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C, 48% activity (purified from tea), 62% activity (recombinant enzyme) Camellia sinensis primeverose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.149 ? x * 61000, SDS-PAGE Camellia sinensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.149 beta-primeverosidase
-
 Camellia sinensis