BRENDA - Enzyme Database

Effect of different phenolic compounds on alpha-amylase activity: screening by microplate-reader based kinetic assay

Funke, I.; Melzig, M.F.; Pharmazie 60, 796-797 (2005)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.2.1.1
medicine
the enzyme is a possible target for treatment of diabetes type 2
Sus scrofa
3.2.1.1
pharmacology
the enzyme is a target for development of specific inhibitors for diabetes type 2 therapy
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.1
acarbose
IC50 is 0.023 mM
Sus scrofa
3.2.1.1
apigenin-7-glucoside
IC50 is 0.17 mM
Sus scrofa
3.2.1.1
caffeic acid
IC50 is 4.8 mM
Sus scrofa
3.2.1.1
chlorogenic acid
IC50 is 1.4 mM
Sus scrofa
3.2.1.1
cynarin
IC50 is above 2.0 mM
Sus scrofa
3.2.1.1
dihydrocaffeic acid
IC50 is above 14.0 mM
Sus scrofa
3.2.1.1
ferulic acid
IC50 is above 5.0 mM
Sus scrofa
3.2.1.1
fisetin
IC50 is 0.44 mM
Sus scrofa
3.2.1.1
isochlorogenic acid
IC50 is 0.56 mM
Sus scrofa
3.2.1.1
luteolin
IC50 is 0.17 mM
Sus scrofa
3.2.1.1
luteolin-7-glucoside
IC50 is 0.28 mM
Sus scrofa
3.2.1.1
quinic acid
IC50 is above 13.0 mM
Sus scrofa
3.2.1.1
rosmarinic acid
IC50 is 1.4 mM
Sus scrofa
3.2.1.1
Sinapic acid
IC50 is above 6.7 mM
Sus scrofa
3.2.1.1
Tannic acid
IC50 is 0.14 mM
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.1
Sus scrofa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.1
commercial preparation
from pancreas
Sus scrofa
-
3.2.1.1
pancreas
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.1
additional information
-
microplate-reader based kinetic assay
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.1
4-nitrophenyl alpha-D-maltopentaoside + H2O
-
666544
Sus scrofa
4-nitrophenol + alpha-D-maltopentaose
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.2.1.1
0.023
-
IC50 is 0.023 mM
Sus scrofa
acarbose
3.2.1.1
0.14
-
IC50 is 0.14 mM
Sus scrofa
Tannic acid
3.2.1.1
0.17
-
IC50 is 0.17 mM
Sus scrofa
apigenin-7-glucoside
3.2.1.1
0.17
-
IC50 is 0.17 mM
Sus scrofa
luteolin
3.2.1.1
0.28
-
IC50 is 0.28 mM
Sus scrofa
luteolin-7-glucoside
3.2.1.1
0.44
-
IC50 is 0.44 mM
Sus scrofa
fisetin
3.2.1.1
0.56
-
IC50 is 0.56 mM
Sus scrofa
isochlorogenic acid
3.2.1.1
1.4
-
IC50 is 1.4 mM
Sus scrofa
chlorogenic acid
3.2.1.1
1.4
-
IC50 is 1.4 mM
Sus scrofa
rosmarinic acid
3.2.1.1
2
-
IC50 is above 2.0 mM
Sus scrofa
cynarin
3.2.1.1
4.8
-
IC50 is 4.8 mM
Sus scrofa
caffeic acid
3.2.1.1
5
-
IC50 is above 5.0 mM
Sus scrofa
ferulic acid
3.2.1.1
6.7
-
IC50 is above 6.7 mM
Sus scrofa
Sinapic acid
3.2.1.1
13
-
IC50 is above 13.0 mM
Sus scrofa
quinic acid
3.2.1.1
14
-
IC50 is above 14.0 mM
Sus scrofa
dihydrocaffeic acid
Application (protein specific)
EC Number
Application
Commentary
Organism
3.2.1.1
medicine
the enzyme is a possible target for treatment of diabetes type 2
Sus scrofa
3.2.1.1
pharmacology
the enzyme is a target for development of specific inhibitors for diabetes type 2 therapy
Sus scrofa
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.2.1.1
0.023
-
IC50 is 0.023 mM
Sus scrofa
acarbose
3.2.1.1
0.14
-
IC50 is 0.14 mM
Sus scrofa
Tannic acid
3.2.1.1
0.17
-
IC50 is 0.17 mM
Sus scrofa
apigenin-7-glucoside
3.2.1.1
0.17
-
IC50 is 0.17 mM
Sus scrofa
luteolin
3.2.1.1
0.28
-
IC50 is 0.28 mM
Sus scrofa
luteolin-7-glucoside
3.2.1.1
0.44
-
IC50 is 0.44 mM
Sus scrofa
fisetin
3.2.1.1
0.56
-
IC50 is 0.56 mM
Sus scrofa
isochlorogenic acid
3.2.1.1
1.4
-
IC50 is 1.4 mM
Sus scrofa
chlorogenic acid
3.2.1.1
1.4
-
IC50 is 1.4 mM
Sus scrofa
rosmarinic acid
3.2.1.1
2
-
IC50 is above 2.0 mM
Sus scrofa
cynarin
3.2.1.1
4.8
-
IC50 is 4.8 mM
Sus scrofa
caffeic acid
3.2.1.1
5
-
IC50 is above 5.0 mM
Sus scrofa
ferulic acid
3.2.1.1
6.7
-
IC50 is above 6.7 mM
Sus scrofa
Sinapic acid
3.2.1.1
13
-
IC50 is above 13.0 mM
Sus scrofa
quinic acid
3.2.1.1
14
-
IC50 is above 14.0 mM
Sus scrofa
dihydrocaffeic acid
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.1
acarbose
IC50 is 0.023 mM
Sus scrofa
3.2.1.1
apigenin-7-glucoside
IC50 is 0.17 mM
Sus scrofa
3.2.1.1
caffeic acid
IC50 is 4.8 mM
Sus scrofa
3.2.1.1
chlorogenic acid
IC50 is 1.4 mM
Sus scrofa
3.2.1.1
cynarin
IC50 is above 2.0 mM
Sus scrofa
3.2.1.1
dihydrocaffeic acid
IC50 is above 14.0 mM
Sus scrofa
3.2.1.1
ferulic acid
IC50 is above 5.0 mM
Sus scrofa
3.2.1.1
fisetin
IC50 is 0.44 mM
Sus scrofa
3.2.1.1
isochlorogenic acid
IC50 is 0.56 mM
Sus scrofa
3.2.1.1
luteolin
IC50 is 0.17 mM
Sus scrofa
3.2.1.1
luteolin-7-glucoside
IC50 is 0.28 mM
Sus scrofa
3.2.1.1
quinic acid
IC50 is above 13.0 mM
Sus scrofa
3.2.1.1
rosmarinic acid
IC50 is 1.4 mM
Sus scrofa
3.2.1.1
Sinapic acid
IC50 is above 6.7 mM
Sus scrofa
3.2.1.1
Tannic acid
IC50 is 0.14 mM
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.1
commercial preparation
from pancreas
Sus scrofa
-
3.2.1.1
pancreas
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.1
additional information
-
microplate-reader based kinetic assay
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.1
4-nitrophenyl alpha-D-maltopentaoside + H2O
-
666544
Sus scrofa
4-nitrophenol + alpha-D-maltopentaose
-
-
-
?