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Literature summary extracted from

  • Kato, Y.; Tanaka, T.; Miyata, T.
    Comparison of kinetic properties of a hydrophilic form of acetylcholinesterase purified from strains susceptible and resistant to carbamate and organophosphorus insecticides of green rice leafhopper (Nephotettix cincticeps Uhler) (2004), Pestic. Biochem. Physiol., 79, 64-73.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 acetylthiocholine N-methyl carbamate-resistant strain, no substrate inhibition up to 0.01 mM Nephotettix cincticeps Uhler
3.1.1.7 butyrylthiocholine N-methyl carbamate-resistant strain, no substrate inhibition up to 0.01 mM Nephotettix cincticeps Uhler
3.1.1.7 Monocrotophos N-methyl carbamate-resistant strain is 65fold more sensitive than susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 propionylthiocholine N-methyl carbamate-resistant strain, no substrate inhibition up to 0.01 mM Nephotettix cincticeps Uhler
3.1.1.7 propoxur N-methyl carbamate-resistant strain is 53fold less sensitive than susceptible strain Nephotettix cincticeps Uhler

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.0391
-
 propionylthiocholine pH 7.5, 25°C, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 0.0461
-
 butyrylthiocholine pH 7.5, 25°C, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 0.0511
-
 acetylthiocholine pH 7.5, 25°C, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 0.0852
-
 butyrylthiocholine pH 7.5, 25°C, N-methyl carbamate-resisitant strain Nephotettix cincticeps Uhler
3.1.1.7 0.0881
-
 propionylthiocholine pH 7.5, 25°C, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler
3.1.1.7 0.0918
-
 acetylthiocholine pH 7.5, 25°C, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Nephotettix cincticeps Uhler
-
 strains susceptible and resistant to carbamate and organophosphorus insecticides
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7
-
 Nephotettix cincticeps Uhler

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 4.6
-
 pH 7.5, 25°C, substrate butyrylthiocholine, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 14.5
-
 pH 7.5, 25°C, substrate butyrylthiocholine, N-methyl carbamate-resisitant strain Nephotettix cincticeps Uhler
3.1.1.7 27
-
 pH 7.5, 25°C, substrate propionylthiocholine, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler
3.1.1.7 30.5
-
 pH 7.5, 25°C, substrate propionylthiocholine, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 70.2
-
 pH 7.5, 25°C, substrate acetylthiocholine, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
3.1.1.7 123
-
 pH 7.5, 25°C, substrate acetylthiocholine, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine + H2O
-
 Nephotettix cincticeps Uhler thiocholine + acetate
-
 ?
3.1.1.7 butyrylthiocholine + H2O
-
 Nephotettix cincticeps Uhler butyrate + thiocholine
-
 ?
3.1.1.7 propionylthiocholine + H2O
-
 Nephotettix cincticeps Uhler propanoate + thiocholine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 8.5
-
 both isoform isolated from strain susceptible and from strain resistant to carbamate and organophosphorus insecticides Nephotettix cincticeps Uhler