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Literature summary extracted from
- Pectate lyase C from Bacillus subtilis: a novel endo-cleaving enzyme with activity on highly methylated pectin (2006), Microbiology, 152, 617-625.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | expression in Escherichia coli | Bacillus subtilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.2 | additional information | - |
additional information | KM-value is 0.13 mg/ml for 22% esterified pectin, the KM-value is 0.15 mg/ml for polygalacturonic acid | Bacillus subtilis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.2 | Ca2+ | absolute requirement | Bacillus subtilis |  |
| 4.2.2.2 | Hg2+ | 1 mM, can replace Ca2+ in activation | Bacillus subtilis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.2 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | recombinant enzyme | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.2 | pectin | of methyl esterification degree from 22% to 89%, maximal activity on 22% esterified citrus pectin | Bacillus subtilis | ? | - |
? | |
| 4.2.2.2 | polygalacturonate | - |
Bacillus subtilis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.2 | pectate lyase C | - |
Bacillus subtilis |
| 4.2.2.2 | pelC | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 65 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 50 | 80 | 50°C: about 60% of maximal activity, 80°C: about 60% of maximal activity | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 10 | - |
- |
Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 9 | 11 | pH 9: about 40% of maximal activity, pH 11: about 50% of maximal activity | Bacillus subtilis |
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