BRENDA - Enzyme Database

Diglycoside-specific glycosidases

Sakata, K.; Mizutani, M.; Ma, S.J.; Hiratake, J.; Methods Enzymol. 363, 444-459 (2003) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.149
-
Camellia sinensis
3.2.1.149
expression in Escherichia coli
Camellia sinensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.149
2
-
2-phenylethyl beta-primeveroside
pH 6.0, 37°C
Camellia sinensis
3.2.1.149
14.9
-
2-phenylethyl beta-vicianoside
pH 6.0, 37°C
Camellia sinensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.149
60170
-
TOFMS analysis
Camellia sinensis
3.2.1.149
60310
-
TOFMS analysis
Camellia sinensis
3.2.1.149
60480
-
TOFMS analysis
Camellia sinensis
3.2.1.149
61000
-
1 * 61000, SDS-PAGE, TOFMS analysis
Camellia sinensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.149
Camellia sinensis
Q7X9A9
var assamica
-
3.2.1.149
Camellia sinensis
Q7X9A9
var sinensis cv Shuixian
-
3.2.1.149
Camellia sinensis
Q7X9A9
var sinensis cv Yabukita
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.149
-
Camellia sinensis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.149
0.9
-
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
Camellia sinensis
3.2.1.149
0.98
-
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
Camellia sinensis
3.2.1.149
0.99
-
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
Camellia sinensis
3.2.1.149
1.7
-
purified enzyme
Camellia sinensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.149
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
pH 6.0, 37°C, 0.05% activity
666247
Camellia sinensis
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
pH 6.0, 37°C, 0.60% activity
666247
Camellia sinensis
beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-gentiobioside + H2O
pH 6.0, 37°C, 0.34% activity
666247
Camellia sinensis
gentiobiose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-primeveroside + H2O
pH 6.0, 37°C
666247
Camellia sinensis
primeverose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-primeveroside + H2O
pH 6.0, 37°C, 100% activity
666247
Camellia sinensis
primeverose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-vicianoside + H2O
pH 6.0, 37°C, 2.97% activity
666247
Camellia sinensis
vicianose + 2-phenylethanol
-
-
-
?
3.2.1.149
p-nitrophenol beta-D-glucopyranoside + H2O
pH 6.0, 37°C, 0.23% activity
666247
Camellia sinensis
D-glucose + p-nitrophenol
-
-
-
?
3.2.1.149
p-nitrophenyl beta-primeveroside + H2O
pH 6.0, 37°C
666247
Camellia sinensis
primeverose + p-nitrophenol
-
-
-
?
3.2.1.149
p-nitrophenyl beta-primeveroside + H2O
pH 6.0, 37°C, 54.4% activity
666247
Camellia sinensis
primeverose + p-nitrophenol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.149
monomer
1 * 61000, SDS-PAGE, TOFMS analysis
Camellia sinensis
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.149
beta-primeverosidase
-
Camellia sinensis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.149
45
-
-
Camellia sinensis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.149
40
-
estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C)
Camellia sinensis
3.2.1.149
45
-
estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C)
Camellia sinensis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.149
5.83
-
2-phenylethyl beta-vicianoside
pH 6.0, 37°C
Camellia sinensis
3.2.1.149
43.8
-
2-phenylethyl beta-primeveroside
pH 6.0, 37°C
Camellia sinensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.149
4
-
-
Camellia sinensis
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.149
3
5
-
Camellia sinensis
3.2.1.149
4
5
-
Camellia sinensis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.149
Camellia sinensis
-
-
9.4
3.2.1.149
Camellia sinensis
-
-
9.5
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.149
-
Camellia sinensis
3.2.1.149
expression in Escherichia coli
Camellia sinensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.149
2
-
2-phenylethyl beta-primeveroside
pH 6.0, 37°C
Camellia sinensis
3.2.1.149
14.9
-
2-phenylethyl beta-vicianoside
pH 6.0, 37°C
Camellia sinensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.149
60170
-
TOFMS analysis
Camellia sinensis
3.2.1.149
60310
-
TOFMS analysis
Camellia sinensis
3.2.1.149
60480
-
TOFMS analysis
Camellia sinensis
3.2.1.149
61000
-
1 * 61000, SDS-PAGE, TOFMS analysis
Camellia sinensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.149
-
Camellia sinensis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.149
0.9
-
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
Camellia sinensis
3.2.1.149
0.98
-
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
Camellia sinensis
3.2.1.149
0.99
-
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
Camellia sinensis
3.2.1.149
1.7
-
purified enzyme
Camellia sinensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.149
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
pH 6.0, 37°C, 0.05% activity
666247
Camellia sinensis
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
pH 6.0, 37°C, 0.60% activity
666247
Camellia sinensis
beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-gentiobioside + H2O
pH 6.0, 37°C, 0.34% activity
666247
Camellia sinensis
gentiobiose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-primeveroside + H2O
pH 6.0, 37°C
666247
Camellia sinensis
primeverose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-primeveroside + H2O
pH 6.0, 37°C, 100% activity
666247
Camellia sinensis
primeverose + 2-phenylethanol
-
-
-
?
3.2.1.149
2-phenylethyl beta-vicianoside + H2O
pH 6.0, 37°C, 2.97% activity
666247
Camellia sinensis
vicianose + 2-phenylethanol
-
-
-
?
3.2.1.149
p-nitrophenol beta-D-glucopyranoside + H2O
pH 6.0, 37°C, 0.23% activity
666247
Camellia sinensis
D-glucose + p-nitrophenol
-
-
-
?
3.2.1.149
p-nitrophenyl beta-primeveroside + H2O
pH 6.0, 37°C
666247
Camellia sinensis
primeverose + p-nitrophenol
-
-
-
?
3.2.1.149
p-nitrophenyl beta-primeveroside + H2O
pH 6.0, 37°C, 54.4% activity
666247
Camellia sinensis
primeverose + p-nitrophenol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.149
monomer
1 * 61000, SDS-PAGE, TOFMS analysis
Camellia sinensis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.149
45
-
-
Camellia sinensis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.149
40
-
estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C)
Camellia sinensis
3.2.1.149
45
-
estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C)
Camellia sinensis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.149
5.83
-
2-phenylethyl beta-vicianoside
pH 6.0, 37°C
Camellia sinensis
3.2.1.149
43.8
-
2-phenylethyl beta-primeveroside
pH 6.0, 37°C
Camellia sinensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.149
4
-
-
Camellia sinensis
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.149
3
5
-
Camellia sinensis
3.2.1.149
4
5
-
Camellia sinensis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.149
Camellia sinensis
-
-
9.4
3.2.1.149
Camellia sinensis
-
-
9.5