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Literature summary extracted from

  • Millership, J.J.; Chappell, C.; Okhuysen, P.C.; Snowden, K.F.
    Characterization of aminopeptidase activity from three species of microsporidia: Encephalitozoon cuniculi, Encephalitozoon hellem, and Vittaforma corneae (2002), J. Parasitol., 88, 843-848.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 86.3% inhibition at 5 mM Encephalitozoon cuniculi
3.4.11.1 1,10-phenanthroline 87.8% inhibition at 5 mM Encephalitozoon hellem
3.4.11.1 bestatin 92.5% inhibition at 0.3 mM Encephalitozoon cuniculi
3.4.11.1 bestatin 87.0% inhibition at 0.3 mM Encephalitozoon hellem
3.4.11.1 additional information no inhibition by epibestatin, and by E64, chymostatin, leupeptin, and antipain Encephalitozoon cuniculi
3.4.11.1 additional information no inhibition by epibestatin, and by E64, chymostatin, leupeptin, and antipain Encephalitozoon hellem
3.4.11.1 nitrobestatin 95.1% inhibition at 0.133 mM Encephalitozoon cuniculi
3.4.11.6 1,10-phenanthroline 68.5% inhibition at 5 mM Vittaforma corneae
3.4.11.6 bestatin 84.8% inhibition at 0.3 mM Vittaforma corneae
3.4.11.6 additional information no inhibition by epibestatin, and by E64, chymostatin, leupeptin, and antipain Vittaforma corneae
3.4.11.6 nitrobestatin 92.3% inhibition at 0.133 mM Vittaforma corneae
3.4.11.22 1,10-phenanthroline 86.3% inhibition at 5 mM Encephalitozoon cuniculi
3.4.11.22 1,10-phenanthroline 87.8% inhibition at 5 mM Encephalitozoon hellem
3.4.11.22 bestatin 92.5% inhibition at 0.3 mM Encephalitozoon cuniculi
3.4.11.22 bestatin 87.0% inhibition at 0.3 mM Encephalitozoon hellem
3.4.11.22 additional information no inhibition by epibestatin, and by E64, chymostatin, leupeptin, and antipain Encephalitozoon cuniculi
3.4.11.22 additional information no inhibition by epibestatin, and by E64, chymostatin, leupeptin, and antipain Encephalitozoon hellem
3.4.11.22 nitrobestatin 95.1% inhibition at 0.133 mM Encephalitozoon cuniculi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytosol
-
 Encephalitozoon cuniculi 5829
-
3.4.11.1 cytosol
-
 Encephalitozoon hellem 5829
-
3.4.11.6 cytosol
-
 Vittaforma corneae 5829
-
3.4.11.22 cytosol
-
 Encephalitozoon cuniculi 5829
-
3.4.11.22 cytosol
-
 Encephalitozoon hellem 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 additional information metallopeptidase Encephalitozoon cuniculi
3.4.11.1 additional information metallopeptidase Encephalitozoon hellem
3.4.11.22 additional information metallopeptidase Encephalitozoon cuniculi
3.4.11.22 additional information metallopeptidase Encephalitozoon hellem

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 72000
-
 native PAGE Encephalitozoon hellem
3.4.11.1 74000
-
 native PAGE Encephalitozoon cuniculi
3.4.11.6 78000
-
 native PAGE Vittaforma corneae
3.4.11.22 72000
-
 native PAGE Encephalitozoon hellem
3.4.11.22 74000
-
 native PAGE Encephalitozoon cuniculi

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Encephalitozoon cuniculi
-
-
-
3.4.11.1 Encephalitozoon hellem
-
-
-
3.4.11.6 Vittaforma corneae
-
-
-
3.4.11.22 Encephalitozoon cuniculi
-
-
-
3.4.11.22 Encephalitozoon hellem
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 partially, preparation of spores and subcellular fractionation Encephalitozoon cuniculi
3.4.11.1 partially, preparation of spores and subcellular fractionation Encephalitozoon hellem
3.4.11.6 partially, preparation of spores and subcellular fractionation Vittaforma corneae
3.4.11.22 partially, preparation of spores and subcellular fractionation Encephalitozoon cuniculi
3.4.11.22 partially, preparation of spores and subcellular fractionation Encephalitozoon hellem

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 spore
-
 Encephalitozoon cuniculi
-
3.4.11.1 spore
-
 Encephalitozoon hellem
-
3.4.11.6 spore
-
 Vittaforma corneae
-
3.4.11.22 spore
-
 Encephalitozoon cuniculi
-
3.4.11.22 spore
-
 Encephalitozoon hellem
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon cuniculi L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon hellem L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon cuniculi L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon hellem L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O best substrate Encephalitozoon cuniculi L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O best substrate Encephalitozoon hellem L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Encephalitozoon cuniculi L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Encephalitozoon hellem L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Phe-7-amido-4-methylcoumarin + H2O low activity Encephalitozoon cuniculi L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Phe-7-amido-4-methylcoumarin + H2O low activity Encephalitozoon hellem L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Vittaforma corneae L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Arg-7-amido-4-methylcoumarin + H2O best substrate Vittaforma corneae L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Vittaforma corneae L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Vittaforma corneae L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Phe-7-amido-4-methylcoumarin + H2O low activity Vittaforma corneae L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon cuniculi L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon hellem L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon cuniculi L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Encephalitozoon hellem L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Leu-7-amido-4-methylcoumarin + H2O best substrate Encephalitozoon cuniculi L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Leu-7-amido-4-methylcoumarin + H2O best substrate Encephalitozoon hellem L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Encephalitozoon cuniculi L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Encephalitozoon hellem L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Phe-7-amido-4-methylcoumarin + H2O low activity Encephalitozoon cuniculi L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Phe-7-amido-4-methylcoumarin + H2O low activity Encephalitozoon hellem L-Phe + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Encephalitozoon cuniculi
3.4.11.1 37
-
 assay at Encephalitozoon hellem
3.4.11.6 37
-
 assay at Vittaforma corneae
3.4.11.22 37
-
 assay at Encephalitozoon cuniculi
3.4.11.22 37
-
 assay at Encephalitozoon hellem