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Literature summary extracted from

  • Kashiwagi, M.; Fuhshuku, K.I.; Sugai, T.
    Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564: preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes (2004), J. Mol. Catal. B, 29, 249-258.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.4 synthesis application in an one-pot preparation of amides from aldehydes together with nitrile hydratase, EC 4.2.1.84 Rhodococcus rhodochrous
4.2.1.84 synthesis production of 2-naphthylacetamide by one-pot chemo-enzymatic conversion Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 NH3 selective product inhibition Rhodococcus rhodochrous

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.4 dimethylformamide the enzyme is still active at 30% v/v Rhodococcus rhodochrous
4.2.1.84 dimethylformamide 30% v/v, no residual activity Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Rhodococcus rhodochrous
-
-
-
3.5.1.4 Rhodococcus rhodochrous IFO 15564
-
-
-
4.2.1.84 Rhodococcus rhodochrous
-
-
-
4.2.1.84 Rhodococcus rhodochrous IFO 15564
-
-
-

Storage Stability

EC Number Storage Stability Organism
4.2.1.84 -20°C, 2 weeks, 90% residual activity, with addition of 8% glycerol, complete loss of activity within one week Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 1-naphthylnitrile + H2O
-
 Rhodococcus rhodochrous 1-naphthylamide
-
 ?
4.2.1.84 1-naphthylnitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 1-naphthylamide
-
 ?
4.2.1.84 2-furonitrile + H2O
-
 Rhodococcus rhodochrous 2-furoamide
-
 ?
4.2.1.84 2-furonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 2-furoamide
-
 ?
4.2.1.84 2-naphthylacetonitrile + H2O
-
 Rhodococcus rhodochrous 2-naphthylacetamide
-
 ?
4.2.1.84 2-naphthylacetonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 2-naphthylacetamide
-
 ?
4.2.1.84 5-hydroxymethyl-2-furonitrile + H2O
-
 Rhodococcus rhodochrous 5-hydroxymethyl-2-furamide
-
 ?
4.2.1.84 5-hydroxymethyl-2-furonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 5-hydroxymethyl-2-furamide
-
 ?
4.2.1.84 benzonitrile + H2O
-
 Rhodococcus rhodochrous benzamide
-
 ?
4.2.1.84 benzonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 benzamide
-
 ?
4.2.1.84 trans-cinnamonitrile + H2O
-
 Rhodococcus rhodochrous trans-cinnamide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 45
-
 assay at Rhodococcus rhodochrous
4.2.1.84 35
-
-
 Rhodococcus rhodochrous

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 additional information
-
 the enzyme shows higher heat tolerance Rhodococcus rhodochrous

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7 8.4
-
 Rhodococcus rhodochrous

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.84 6 9
-
 Rhodococcus rhodochrous