BRENDA - Enzyme Database

Unique regulation of the active site of the serine esterase S-formylglutathione hydrolase

Cummins, I.; McAuley, K.; Fordham-Skelton, A.; Schwoerer, R.; Steel, P.G.; Davis, B.G.; Edwards, R.; J. Mol. Biol. 359, 422-432 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.12
expression of His-tagged wild-type and mutant enzymes in Escherichia coli and in Arabidopsis thaliana protoplasts
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.12
C59S
site-directed mutagenesis, the mutant shows no hydrolase activity with S-formylglutathione, but with 4-methylumbelliferyl acetate in contrast to the wild-type enzyme
Arabidopsis thaliana
3.1.2.12
S152A
site-directed mutagenesis, completely inactive mutant
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
glutathione
reversible disulfide formation at Cys59, regulatory function
Arabidopsis thaliana
3.1.2.12
iodoacetamide
-
Arabidopsis thaliana
3.1.2.12
additional information
covalent modification of Cys59 inhibits the enzyme completely
Arabidopsis thaliana
3.1.2.12
NEM
-
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
32586
-
x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.12
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel chelate affinity chromatography
Arabidopsis thaliana
Reaction
EC Number
Reaction
Commentary
Organism
3.1.2.12
S-formylglutathione + H2O = glutathione + formate
Cys59 and Ser152 are involved in catalysis
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
additional information
the enzyme is a serine hydrolase rather than a cysteine hydrolase
666081
Arabidopsis thaliana
?
-
-
-
-
3.1.2.12
S-formylglutathione + H2O
-
666081
Arabidopsis thaliana
glutathione + formate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
?
x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry
Arabidopsis thaliana
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
25
-
assay at
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
7.2
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
expression of His-tagged wild-type and mutant enzymes in Escherichia coli and in Arabidopsis thaliana protoplasts
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.12
C59S
site-directed mutagenesis, the mutant shows no hydrolase activity with S-formylglutathione, but with 4-methylumbelliferyl acetate in contrast to the wild-type enzyme
Arabidopsis thaliana
3.1.2.12
S152A
site-directed mutagenesis, completely inactive mutant
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
glutathione
reversible disulfide formation at Cys59, regulatory function
Arabidopsis thaliana
3.1.2.12
iodoacetamide
-
Arabidopsis thaliana
3.1.2.12
additional information
covalent modification of Cys59 inhibits the enzyme completely
Arabidopsis thaliana
3.1.2.12
NEM
-
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
32586
-
x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry
Arabidopsis thaliana
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel chelate affinity chromatography
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
additional information
the enzyme is a serine hydrolase rather than a cysteine hydrolase
666081
Arabidopsis thaliana
?
-
-
-
-
3.1.2.12
S-formylglutathione + H2O
-
666081
Arabidopsis thaliana
glutathione + formate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
?
x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
25
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
7.2
-
assay at
Arabidopsis thaliana