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Literature summary extracted from

  • Cummins, I.; McAuley, K.; Fordham-Skelton, A.; Schwoerer, R.; Steel, P.G.; Davis, B.G.; Edwards, R.
    Unique regulation of the active site of the serine esterase S-formylglutathione hydrolase (2006), J. Mol. Biol., 359, 422-432.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.12 expression of His-tagged wild-type and mutant enzymes in Escherichia coli and in Arabidopsis thaliana protoplasts Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.12 C59S site-directed mutagenesis, the mutant shows no hydrolase activity with S-formylglutathione, but with 4-methylumbelliferyl acetate in contrast to the wild-type enzyme Arabidopsis thaliana
3.1.2.12 S152A site-directed mutagenesis, completely inactive mutant Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.12 glutathione reversible disulfide formation at Cys59, regulatory function Arabidopsis thaliana
3.1.2.12 iodoacetamide
-
 Arabidopsis thaliana
3.1.2.12 additional information covalent modification of Cys59 inhibits the enzyme completely Arabidopsis thaliana
3.1.2.12 NEM
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.12 32586
-
 x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.12 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.12 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel chelate affinity chromatography Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.12 S-formylglutathione + H2O = glutathione + formate Cys59 and Ser152 are involved in catalysis Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.12 additional information the enzyme is a serine hydrolase rather than a cysteine hydrolase Arabidopsis thaliana ?
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Arabidopsis thaliana glutathione + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.12 ? x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.2.12 esterase D
-
 Arabidopsis thaliana
3.1.2.12 serine esterase
-
 Arabidopsis thaliana
3.1.2.12 SFGH
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.12 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.12 7.2
-
 assay at Arabidopsis thaliana