EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.11 | expression in insect SF9 cells | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.26.11 | A493T | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | A501T | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | D502T | processing efficiency reduced 7420fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | G506A | processing efficiency reduced 11fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | H498A | processing efficiency reduced 1950fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | H500A | processing efficiency reduced 3379fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | H503A | processing efficiency reduced 817fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | H504A | processing efficiency reduced 7fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | I494A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | I505A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | I508A | processing efficiency reduced 6fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | L491A | processing efficiency reduced 7fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | L499A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | L507A | processing efficiency reduced 6fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | Q492A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | S497A | processing efficiency reduced 495fold compared to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | V496A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
3.1.26.11 | Y495A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.11 | 0.000155 | - |
pre-tRNAHis | 25 mM Tris-HCl pH 7.0, 1 mM MgCl2, 1 mM DTT, 50 mM KCl at 28°C | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.26.11 | 82920 | - |
calculated from sequence | Drosophila melanogaster |
3.1.26.11 | 90000 | - |
SDS-PAGE | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.11 | Drosophila melanogaster | Q8MKW7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.11 | His-tag fusion, Ni-affinity chromatography | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.26.11 | cell culture | - |
Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.11 | pre-tRNAHis + H2O | - |
Drosophila melanogaster | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.11 | monomer | 1 * 90000, SDS-PAGE | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.11 | tRNaseZ | - |
Drosophila melanogaster |