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Literature summary extracted from

  • Garau, G.; Bebrone, C.; Anne, C.; Galleni, M.; Frere, J.M.; Dideberg, O.
    A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem (2005), J. Mol. Biol., 345, 785-795.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 crystal structure of wild-type enzyme mutant enzyme N220G, and of the complex of wild-type enzyme with biapenem Aeromonas hydrophila

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 N220G kinetic parameters of the wild-type and N220G mutant enzymes are not significantly different Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.05
-
 Imipenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
3.5.2.6 0.118
-
 biapenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
3.5.2.6 0.166
-
 biapenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila
3.5.2.6 0.34
-
 Imipenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zinc enzyme contains one zinc ion Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Aeromonas hydrophila P26918
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 biapenem + H2O
-
 Aeromonas hydrophila (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
3.5.2.6 imipenem + H2O
-
 Aeromonas hydrophila (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 carbapenemase CphA
-
 Aeromonas hydrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 160
-
 biapenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
3.5.2.6 300
-
 biapenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila
3.5.2.6 390
-
 Imipenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
3.5.2.6 1200
-
 Imipenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila