BRENDA - Enzyme Database

Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites

Ehinger, S.; Schubert, W.D.; Bergmann, S.; Hammerschmidt, S.; Heinz, D.W.; J. Mol. Biol. 343, 997-1005 (2004)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.11
molecular biology
alpha-enolase doubles as a surface-displayed plasminogen-binder supporting virulence
Streptococcus pneumoniae
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Streptococcus pneumoniae
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
2.0 A resolution, molecular replacement, hanging-drop vapor-diffusion
Streptococcus pneumoniae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
deltaK433/K434
mutant with different oligomerization state
Streptococcus pneumoniae
4.2.1.11
K433L/K434L
mutant with different oligomerization state
Streptococcus pneumoniae
4.2.1.11
K434L
mutant with different oligomerization state
Streptococcus pneumoniae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
-
Streptococcus pneumoniae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
370000
-
gel filtration
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Streptococcus pneumoniae
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Streptococcus pneumoniae
Q97QS2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
ion exchange chromatography and gel filtration
Streptococcus pneumoniae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
111
-
room temperature, pH 7.5, MgCl2, KCl, 2-phosphoglyceric acid
Streptococcus pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666033
Streptococcus pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
-
Streptococcus pneumoniae
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.11
molecular biology
alpha-enolase doubles as a surface-displayed plasminogen-binder supporting virulence
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Streptococcus pneumoniae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
2.0 A resolution, molecular replacement, hanging-drop vapor-diffusion
Streptococcus pneumoniae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
deltaK433/K434
mutant with different oligomerization state
Streptococcus pneumoniae
4.2.1.11
K433L/K434L
mutant with different oligomerization state
Streptococcus pneumoniae
4.2.1.11
K434L
mutant with different oligomerization state
Streptococcus pneumoniae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
-
Streptococcus pneumoniae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
370000
-
gel filtration
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Streptococcus pneumoniae
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
ion exchange chromatography and gel filtration
Streptococcus pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
111
-
room temperature, pH 7.5, MgCl2, KCl, 2-phosphoglyceric acid
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666033
Streptococcus pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
-
Streptococcus pneumoniae