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Literature summary extracted from

  • Ehinger, S.; Schubert, W.D.; Bergmann, S.; Hammerschmidt, S.; Heinz, D.W.
    Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites (2004), J. Mol. Biol., 343, 997-1005.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.11 molecular biology alpha-enolase doubles as a surface-displayed plasminogen-binder supporting virulence Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expression in Escherichia coli Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.11 2.0 A resolution, molecular replacement, hanging-drop vapor-diffusion Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 deltaK433/K434 mutant with different oligomerization state Streptococcus pneumoniae
4.2.1.11 K433L/K434L mutant with different oligomerization state Streptococcus pneumoniae
4.2.1.11 K434L mutant with different oligomerization state Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+
-
 Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 370000
-
 gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Streptococcus pneumoniae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Streptococcus pneumoniae Q97QS2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 ion exchange chromatography and gel filtration Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 111
-
 room temperature, pH 7.5, MgCl2, KCl, 2-phosphoglyceric acid Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Streptococcus pneumoniae phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 octamer
-
 Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 plasminogen-binding alpha-enolase
-
 Streptococcus pneumoniae