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Literature summary extracted from

  • Deejing, S.; Yoshimune, K.; Lumyong, S.; Moriguchi, M.
    Purification and characterization of hyperthermotolerant leucine aminopeptidase from Geobacillus thermoleovorans 47b (2005), J. Ind. Microbiol. Biotechnol., 32, 269-276.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.10 1,10-phenanthroline
-
 Geobacillus thermoleovorans
3.4.11.10 2-mercaptoethanol
-
 Geobacillus thermoleovorans
3.4.11.10 bestatin
-
 Geobacillus thermoleovorans
3.4.11.10 DTT
-
 Geobacillus thermoleovorans
3.4.11.10 EDTA
-
 Geobacillus thermoleovorans
3.4.11.10 iodoacetate
-
 Geobacillus thermoleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 0.658
-
 L-Leu-4-nitroanilide pH 7.6-7.8, 60°C Geobacillus thermoleovorans
3.4.11.10 25
-
 L-Leu-Gly-Gly pH 7.6-7.8, 60°C Geobacillus thermoleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Co2+ reactivates the apoenzyme Geobacillus thermoleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 42977
-
 1 * 42977, mass spectrometry Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Geobacillus thermoleovorans
-
-
-
3.4.11.10 Geobacillus thermoleovorans 47b
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10 native enzyme 164fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydroxyapatite chromatography, again ion exchange chromatography, and gel filtration, followed by again ion exchange and hydroxyapatite chromatography Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.10 additional information
-
-
 Geobacillus thermoleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Arg-4-nitroanilide + H2O
-
 Geobacillus thermoleovorans L-Arg + 4-nitroaniline
-
 ?
3.4.11.10 L-Arg-4-nitroanilide + H2O
-
 Geobacillus thermoleovorans 47b L-Arg + 4-nitroaniline
-
 ?
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Geobacillus thermoleovorans L-Leu + 4-nitroaniline
-
 ?
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Geobacillus thermoleovorans 47b L-Leu + 4-nitroaniline
-
 ?
3.4.11.10 L-Leu-Gly-Gly + H2O
-
 Geobacillus thermoleovorans L-Leu + Gly-Gly
-
 ?
3.4.11.10 L-Leu-Gly-Gly + H2O
-
 Geobacillus thermoleovorans 47b L-Leu + Gly-Gly
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.10 monomer 1 * 42977, mass spectrometry Geobacillus thermoleovorans

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 LAP
-
 Geobacillus thermoleovorans
3.4.11.10 leucine aminopeptidase
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 60
-
-
 Geobacillus thermoleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.10 90
-
 1 h, stable up to Geobacillus thermoleovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 10180
-
 L-Leu-4-nitroanilide pH 7.6-7.8, 60°C Geobacillus thermoleovorans
3.4.11.10 49540
-
 L-Leu-Gly-Gly pH 7.6-7.8, 60°C Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 7.6 7.8
-
 Geobacillus thermoleovorans