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Literature summary extracted from

  • Gilbert, E.J.; Cornish, A.; Jones, C.W.
    Purification and properties of extracellular lipase from Pseudomonas aeruginosa EF2 (1991), J. Gen. Microbiol., 137, 2223-2229.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.3 the purified enzyme is very stable to freezing and thawing Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 3,4-dichloroisocoumarin weak inhibition Pseudomonas aeruginosa
3.1.1.3 additional information no inhibition by EDTA at 1 mM Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 additional information
-
 the enzyme tends to form aggregates Pseudomonas aeruginosa
3.1.1.3 29000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa
-
-
-
3.1.1.3 Pseudomonas aeruginosa EF2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 from medium of a Tween 80-limited culture, by anion exchange chromatography and gel filtration Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates Pseudomonas aeruginosa ?
-
 ?
3.1.1.3 additional information the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates Pseudomonas aeruginosa EF2 ?
-
 ?
3.1.1.3 triolein + H2O olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues Pseudomonas aeruginosa diolein + oleate
-
 ?
3.1.1.3 triolein + H2O olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues Pseudomonas aeruginosa EF2 diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 29000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extracellular lipase
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 60
-
 half-life is 17.5 min Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.3 3000
-
 triolein
-
 Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Pseudomonas aeruginosa
-
-
 4.9