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Literature summary extracted from

  • Fucinos, P.; Abadin, C.M.; Sanroman, A.; Longo, M.A.; Pastrana, L.; Rua, M.L.
    Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterisation (2005), J. Biotechnol., 117, 233-241.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Thermus thermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 34000
-
 x * 34000, SDS-PAGE Thermus thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 isopropanol 4 h, completely stable in 80% at 30°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Thermus thermophilus
-
-
-
3.1.1.3 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 extracellular enzymes partially over 4.1fold by ammonium sulfate and sodium cholate treatment, ethanol/ether precipitation, and hydrophobic interaction chromatography Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.076
-
 partially purified enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Thermus thermophilus 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 4-nitrophenol + laurate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 oligomer x * 34000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 BTL2 lipase
-
 Thermus thermophilus
3.1.1.3 lipase
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 80
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 40 90
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 85
-
 partially purified enzyme, stable Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 10
-
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5 10 and above, activity progressively increases from pH 5.0 to pH 10.0 Thermus thermophilus