Any feedback?
Literature summary extracted from
- Purification and characterization of extracellular 1,2-alpha-L-fucosidase from Bacillus cereus (2005), J. Biosci. Bioeng., 99, 629-635.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.63 | maltose | 10 mM, 10% activation | Bacillus cereus |  |
| 3.2.1.63 | NEM | 1 mM, 20% activation | Bacillus cereus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.63 | BaCl2 | 1 mM, 63% inhibition | Bacillus cereus | |
| 3.2.1.63 | CaCl2 | 1 mM, 60% inhibition | Bacillus cereus | |
| 3.2.1.63 | CoCl2 | 1 mM, 38% inhibition | Bacillus cereus | |
| 3.2.1.63 | CuSO4 | 1 mM, 86% inhibition | Bacillus cereus | |
| 3.2.1.63 | cysteine | 1 mM, 27% inhibition | Bacillus cereus | |
| 3.2.1.63 | D-mannose | 10 mM, 22% inhibition | Bacillus cereus | |
| 3.2.1.63 | dithiothreitol | 1 mM, 22% inhibition | Bacillus cereus | |
| 3.2.1.63 | EDTA | 1 mM, 23% inhibition | Bacillus cereus | |
| 3.2.1.63 | FeSO4 | 1 mM, complete inhibition | Bacillus cereus | |
| 3.2.1.63 | glutathione | 1 mM, 21% inhibition | Bacillus cereus | |
| 3.2.1.63 | Hg2+ | 0.1 mM, complete inhibition | Bacillus cereus | |
| 3.2.1.63 | L-fucose | 10 mM, 91% inhibition | Bacillus cereus | |
| 3.2.1.63 | lactose | 10 mM, 27% inhibition | Bacillus cereus | |
| 3.2.1.63 | NEM | 0.1 mM, 24% inhibition | Bacillus cereus | |
| 3.2.1.63 | NiSO4 | 1 mM, 59% inhibition | Bacillus cereus | |
| 3.2.1.63 | p-mercuribenzoate | 1 mM, 23% inhibition | Bacillus cereus | |
| 3.2.1.63 | ZnCl2 | 1 mM, 43% inhibition | Bacillus cereus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.63 | extracellular | - |
Bacillus cereus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 81000 | - |
2 * 81000, SDS-PAGE | Bacillus cereus |
| 3.2.1.63 | 196000 | - |
gel filtration | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.63 | additional information | Bacillus cereus | inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.63 | Bacillus cereus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.63 | - |
Bacillus cereus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.63 | culture medium | inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including glucose | Bacillus cereus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 51.1 | - |
- |
Bacillus cereus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.63 | Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc + H2O | i.e. lacto-N-fucopentaose I, at 29% of the activity with 2'-fucosyllactose | Bacillus cereus | L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
| 3.2.1.63 | Fucalpha(1-2)Galbeta(1-4)Glc + H2O | i.e. 2'-fucosyllactose | Bacillus cereus | L-fucose + Galbeta(1-4)Glc | - |
? | |
| 3.2.1.63 | Galbeta(1-4)(Fucalpha(1-3))Glc + H2O | i.e. 3-fucosyllactose, at 0.85% of the activity with 2'-fucosyllactose | Bacillus cereus | ? | - |
? | |
| 3.2.1.63 | additional information | inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose | Bacillus cereus | ? | - |
? | |
| 3.2.1.63 | porcine gastric mucin + H2O | at 33% of the activity with 2'-fucosyllactose | Bacillus cereus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.63 | dimer | 2 * 81000, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 50 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 20 | 60 | 20°C: about 45% of maximal activity, 60°C: about 45% of maximal activity | Bacillus cereus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 50 | - |
pH 7.0, 10 min, stable up to | Bacillus cereus |
| 3.2.1.63 | 60 | - |
pH 7.0, 10 min, about 80% loss of activity | Bacillus cereus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 7 | - |
- |
Bacillus cereus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity | Bacillus cereus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 5 | 9 | 37°C, 6 h, stable | Bacillus cereus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.63 | Bacillus cereus | isoelectric focusing | - |
3 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
