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Literature summary extracted from
- Novel bifidobacterial glycosidases acting on sugar chains of mucin glycoproteins (2005), J. Biosci. Bioeng., 99, 457-465.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.63 | - |
Bifidobacterium bifidum |
| 3.2.1.97 | expression in Escherichia coli | Bifidobacterium longum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.97 | 0.165 | - |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | pH 5.0, 37°C | Bifidobacterium longum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.97 | cell surface | - |
Bifidobacterium longum | 9986 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 205000 | - |
x * 205000, calculated from sequence | Bifidobacterium bifidum |
| 3.2.1.97 | 210000 | - |
predicted from amino acid sequence | Bifidobacterium longum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium bifidum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium breve | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium longum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium breve JCM 1192 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium bifidum ATCC 29521 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium longum JCM 7054 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium longum JCM 1217 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium bifidum JCM 7004 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium bifidum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium breve | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium longum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium breve JCM 1192 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium bifidum ATCC 29521 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium longum JCM 7054 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium longum JCM 1217 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium bifidum JCM 7004 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.63 | Bifidobacterium bifidum | Q6JV24 | strain JCM1254 | - |
| 3.2.1.97 | Bifidobacterium bifidum | - |
ATCC29521 | - |
| 3.2.1.97 | Bifidobacterium bifidum | - |
JCM 1254 | - |
| 3.2.1.97 | Bifidobacterium bifidum | - |
JCM 7004 | - |
| 3.2.1.97 | Bifidobacterium bifidum ATCC 29521 | - |
ATCC29521 | - |
| 3.2.1.97 | Bifidobacterium bifidum JCM 7004 | - |
JCM 7004 | - |
| 3.2.1.97 | Bifidobacterium breve | - |
JCM 1192 | - |
| 3.2.1.97 | Bifidobacterium breve JCM 1192 | - |
JCM 1192 | - |
| 3.2.1.97 | Bifidobacterium longum | - |
JCM 7054 | - |
| 3.2.1.97 | Bifidobacterium longum | Q3T552 | JCM 1217 | - |
| 3.2.1.97 | Bifidobacterium longum JCM 1217 | Q3T552 | JCM 1217 | - |
| 3.2.1.97 | Bifidobacterium longum JCM 7054 | - |
JCM 7054 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.97 | recombinant enzyme | Bifidobacterium longum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.63 | 2'-L-fucosyllactose + H2O | reaction with recombinant Fuc domain | Bifidobacterium bifidum | L-fucose + lactose | - |
? | |
| 3.2.1.63 | blood group substance H + H2O | 74% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain | Bifidobacterium bifidum | ? | - |
? | |
| 3.2.1.63 | lacto-N-fucopentaose I + H2O | 61% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain | Bifidobacterium bifidum | ? | - |
? | |
| 3.2.1.63 | additional information | the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B | Bifidobacterium bifidum | ? | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium bifidum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium breve | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium longum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium breve JCM 1192 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium bifidum ATCC 29521 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium longum JCM 7054 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium longum JCM 1217 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium bifidum JCM 7004 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium bifidum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium breve | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium longum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium breve JCM 1192 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium bifidum ATCC 29521 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium longum JCM 7054 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium longum JCM 1217 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium bifidum JCM 7004 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
| 3.2.1.97 | Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum | p-nitrophenol + Gal-beta-1,3-GalNAc | - |
? | |
| 3.2.1.97 | Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum JCM 7054 | p-nitrophenol + Gal-beta-1,3-GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium bifidum | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium breve | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium breve JCM 1192 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium bifidum ATCC 29521 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum JCM 1217 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium bifidum JCM 7004 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
| 3.2.1.97 | additional information | Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | Bifidobacterium longum | ? | - |
? | |
| 3.2.1.97 | additional information | Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | Bifidobacterium longum JCM 1217 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.63 | ? | x * 205000, calculated from sequence | Bifidobacterium bifidum |
| 3.2.1.97 | ? | x * 210000, SDS-PAGE | Bifidobacterium longum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.63 | AfcA protein | - |
Bifidobacterium bifidum |
| 3.2.1.97 | endo-alpha-GalNAc-ase | - |
Bifidobacterium bifidum |
| 3.2.1.97 | endo-alpha-GalNAc-ase | - |
Bifidobacterium breve |
| 3.2.1.97 | endo-alpha-GalNAc-ase | - |
Bifidobacterium longum |
| 3.2.1.97 | endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium bifidum |
| 3.2.1.97 | endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium breve |
| 3.2.1.97 | endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium longum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.97 | 60 | - |
- |
Bifidobacterium longum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 35 | - |
30 min, Fuc domain is stable below | Bifidobacterium bifidum |
| 3.2.1.97 | 37 | - |
the enzyme is stable up to 37°C and retains 73% activity after incubation at 45°C for 30 min in 50 mM acetate buffer (pH 5.0) | Bifidobacterium longum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.97 | 37 | - |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | pH 5.0, 37°C | Bifidobacterium longum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.97 | 5 | - |
at 37°C in 10 mM acetate buffer | Bifidobacterium longum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.97 | 4 | 6 | 68% activity at pH 4.0, 73% activity at pH 6.0 | Bifidobacterium longum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.63 | 6.5 | 7.5 | Fuc domain is stable for 12 h | Bifidobacterium bifidum |
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