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Literature summary extracted from

  • Turner, P.; Labes, A.; Fridjonsson, O.H.; Hreggvidson, G.O.; Schoenheit, P.; Kristjansson, J.K.; Holst, O.; Karlsson, E.N.
    Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria have similar domain structures but differ in oligomeric state and activity profile (2005), J. Biosci. Bioeng., 100, 380-390.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression in Escherichia coli Laceyella sacchari
3.2.1.54 expression in Escherichia coli Anoxybacillus flavithermus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 0.28
-
 alpha-cyclodextrin pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 0.32
-
 beta-cyclodextrin pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 5
-
 soluble starch pH 6, 55°C Laceyella sacchari
3.2.1.54 7.9
-
 alpha-cyclodextrin pH 6, 55°C Laceyella sacchari
3.2.1.54 11
-
 soluble starch pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 12
-
 beta-cyclodextrin pH 6, 55°C Laceyella sacchari

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 71000
-
 2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE Laceyella sacchari
3.2.1.54 71000
-
 2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE Anoxybacillus flavithermus
3.2.1.54 148000
-
 gel filtration Laceyella sacchari
3.2.1.54 148000
-
 gel filtration Anoxybacillus flavithermus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Anoxybacillus flavithermus Q5BLZ6
-
-
3.2.1.54 Laceyella sacchari
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54
-
 Laceyella sacchari
3.2.1.54
-
 Anoxybacillus flavithermus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
 Laceyella sacchari D-glucose + maltose
-
 ?
3.2.1.54 alpha-cyclodextrin + H2O preferred substrate. As compared with the dimer protein, the monomeric enzyme retains only 23% of the specific activity Anoxybacillus flavithermus D-glucose + maltose + maltotriose + maltotetraose + maltoheptaose at lower amounts ?
3.2.1.54 amylose + H2O
-
 Laceyella sacchari ?
-
 ?
3.2.1.54 amylose + H2O
-
 Anoxybacillus flavithermus ?
-
 ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Anoxybacillus flavithermus D-glucose + maltose + maltotriose + maltotetraose + maltoheptaose at lower amounts ?
3.2.1.54 beta-cyclodextrin + H2O preferred substrate Laceyella sacchari D-glucose + maltose
-
 ?
3.2.1.54 pullulan + H2O
-
 Laceyella sacchari ?
-
 ?
3.2.1.54 pullulan + H2O
-
 Anoxybacillus flavithermus ?
-
 ?
3.2.1.54 soluble starch + H2O
-
 Laceyella sacchari ?
-
 ?
3.2.1.54 soluble starch + H2O
-
 Anoxybacillus flavithermus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 dimer 2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE Laceyella sacchari
3.2.1.54 dimer 2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE Anoxybacillus flavithermus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 AfCda13
-
 Anoxybacillus flavithermus
3.2.1.54 LsCda13
-
 Laceyella sacchari

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 57
-
-
 Laceyella sacchari
3.2.1.54 57.5
-
-
 Anoxybacillus flavithermus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 45 70 45°C: about 50% of maximal activity, 70°C: about 70% of maximal activitry Anoxybacillus flavithermus
3.2.1.54 52 60 52°C: about 50% of maximal activity, 60°C: about 30% of maximal activitry Laceyella sacchari

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 57
-
 complete loss of activity after 20 min without calcium, completely stable after 120 min in presence of 1 mM calcium Laceyella sacchari
3.2.1.54 60
-
 half-life: 20 min. Thermal inactivation is not significantly affected by addition of calcium Anoxybacillus flavithermus
3.2.1.54 60
-
 half-life: 4 min, in absence of calcium. 50% loss of activity after 120 min in presence of 1 mM calcium Laceyella sacchari
3.2.1.54 67
-
 complete loss of activity despite presence of calcium Laceyella sacchari

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.54 2.2
-
 beta-cyclodextrin pH 6, 55°C Laceyella sacchari
3.2.1.54 3.6
-
 pullulan pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 13.4
-
 amylose pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 16.9
-
 soluble starch pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 23
-
 amylose pH 6, 55°C Laceyella sacchari
3.2.1.54 36.8
-
 soluble starch pH 6, 55°C Laceyella sacchari
3.2.1.54 42.6
-
 pullulan pH 6, 55°C Laceyella sacchari
3.2.1.54 46.1
-
 alpha-cyclodextrin pH 6, 55°C Laceyella sacchari
3.2.1.54 73.7
-
 beta-cyclodextrin pH 6, 55°C Laceyella sacchari
3.2.1.54 315
-
 beta-cyclodextrin pH 6.5, 55°C Anoxybacillus flavithermus
3.2.1.54 545
-
 alpha-cyclodextrin pH 6.5, 55°C Anoxybacillus flavithermus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6
-
-
 Laceyella sacchari
3.2.1.54 6.5
-
-
 Anoxybacillus flavithermus