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Literature summary extracted from

  • Raux-Deery, E.; Leech, H.K.; Nakrieko, K.A.; McLean, K.J.; Munro, A.W.; Heathcote, P.; Rigby, S.E.; Smith, A.G.; Warren, M.J.
    Identification and characterization of the terminal enzyme of siroheme biosynthesis from Arabidopsis thaliana: a plastid-located sirohydrochlorin ferrochelatase containing a 2FE-2S center (2005), J. Biol. Chem., 280, 4713-4721.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.4 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.99.1.4 Co2+ inactivates the enzyme at high concentrations Arabidopsis thaliana
4.99.1.4 Fe2+ inactivates the enzyme at high concentrations Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.99.1.4 additional information
-
 additional information it is not possible to measure an accurate KM value for the metals as at low concentration of metal the assay is not sensitive enough, whereas at higher metal ion concentrations the enzyme is inactivated Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.99.1.4 chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.99.1.4 Co2+ inactivates the enzyme at high concentrations Arabidopsis thaliana
4.99.1.4 Fe2+ inactivates the enzyme at high concentrations Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.4 sirohydrochlorin + Fe2+ Arabidopsis thaliana
-
 siroheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.4 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.4 recombinant protein Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.99.1.4 0.0054
-
 isolated recombinant enzyme, with ferrous iron as substrate Arabidopsis thaliana
4.99.1.4 0.0485
-
 isolated recombinant enzyme, with cobalt as substrate Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.4 sirohydrochlorin + Co2+ pH 8.0 Arabidopsis thaliana cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+
-
 Arabidopsis thaliana siroheme + H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ pH 8.0 Arabidopsis thaliana siroheme + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.99.1.4 sirohydrochlorin ferrochelatase
-
 Arabidopsis thaliana