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Literature summary extracted from

  • Chow, F.A.; Anderson, K.A.; Noeldner, P.K.; Means, A.R.
    The autonomous activity of calcium/calmodulin-dependent protein kinase IV is required for its role in transcription (2005), J. Biol. Chem., 280, 20530-20538.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 Calmodulin dependent on, activates the nonphosphorylated CaMKIV, residues F320, N321, D322, D323, N318, N324, N330, and N336 are involved in binding, the autoinhibitory domain harbors the binding site for Ca2+ and calmodulin Homo sapiens
2.7.11.17 ionomycin activates nonphosphorylated CaMKIV by increasing intracellular Ca2+ concentration Homo sapiens
2.7.11.17 additional information Ca2+/calmodulin-bound CaMKIV is further activated by phosphorylation through Ca2+/calmodulin-dependent protein kinase kinase CaMKKbeta, which renders CaMKIV independent on Ca2+ and calmodulin, autonomous activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.17 expression of FLAG-tagged CaMKIV in 293A cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.17 F320D/N321D site-directed mutagenesis, the mutation changes the sequence FNDD to DDDD, the mutant shows no binding of calmodulin and increased activity in nonphosphorylated status compared to the wild-type enzyme Homo sapiens
2.7.11.17 additional information mutation of residues N300, N306, N312, N318, N324, N330, N336, N341, and N347 for determination of residues involved in calmodulin binding, no binding of calmodulin and increased activity compared to the wild-type enzyme by nonphosphorylated mutants of N318, N324, N330, and N336, overview Homo sapiens
2.7.11.17 T200A site-directed mutagenesis, the mutant CaMKIV is not phosphorylated by CaMKKbeta and is thus incapable of developing autonomous activity and control gene transcription, the same effect occurs due to combination of T200A with calmodulin binding site mutations, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.17 additional information CaMKIV contains an autoinhibitory domain which harbors the binding site for Ca2+ and calmodulin Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.17 additional information
-
 additional information kinetics for binding of calmodulin Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ dependent on, activates the nonphosphorylated CaMKIV, the autoinhibitory domain harbors the binding site for Ca2+ and calmodulin Homo sapiens
2.7.11.17 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.17 additional information Homo sapiens the enzyme is regulated by Ca2+/calmodulin binding and reversible phosphorylation, overview, the autonomous, Ca2+/calmodulin-independent activity of autophosphorylated Ca2+/calmodulin-dependent protein kinase IV is required for its role in transcription ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.17 phosphoprotein Ca2+/calmodulin-bound CaMKIV is further activated by phosphorylation at Thr200 through Ca2+/calmodulin-dependent protein kinase kinase CaMKKbeta, which renders CaMKIV independent on Ca2+ and calmodulin, autonomous activity, deactivation by dephosphorylation through PP2A Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 recombinant FLAG-tagged CaMKIV from 293A cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.17 0.004 0.022 recombinant nonphosphorylated, calmodulin-binding-residue mutants Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 additional information the enzyme is regulated by Ca2+/calmodulin binding and reversible phosphorylation, overview, the autonomous, Ca2+/calmodulin-independent activity of autophosphorylated Ca2+/calmodulin-dependent protein kinase IV is required for its role in transcription Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.17 calcium/calmodulin-dependent protein kinase IV
-
 Homo sapiens
2.7.11.17 CaMKIV
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.17 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 ATP
-
 Homo sapiens