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Literature summary extracted from

  • Brader, G.; Sjoblom, S.; Hyytiainen, H.; Sims-Huopaniemi, K.; Palva, E.T.
    Altering substrate chain length specificity of an acylhomoserine lactone synthase in bacterial communication (2005), J. Biol. Chem., 280, 10403-10409.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.184 gene expISCC1, expression of wild-type and mutant enzymes in Escherichai coli, inoculation of Solanum tuberosum tubers Pectobacterium carotovorum subsp. carotovorum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.184 F69L site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme Pectobacterium carotovorum subsp. carotovorum
2.3.1.184 M127T site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme Pectobacterium carotovorum subsp. carotovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.184 S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] Pectobacterium carotovorum subsp. carotovorum substrate of wild-type ExpISCC1, specific for 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + 3-oxooctanoyl-[acyl-carrier protein] Pectobacterium carotovorum subsp. carotovorum substrate of wild-type ExpISCC3065, specific for 5'-methylthioadenosine + N-3-oxooctanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.184 Pectobacterium carotovorum subsp. carotovorum Q6R8K9 i.e. Pectobacterium carotovora ssp. carotovora, genes expISCC1 and expISCC3065
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.184 an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone molecular basis of acyl chain length specificity Pectobacterium carotovorum subsp. carotovorum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.184 additional information substrate specificities of wild-type and mutant ExpISCC1 and ExpISCC3065 enzymes Pectobacterium carotovorum subsp. carotovorum ?
-
 ?
2.3.1.184 S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] substrate of wild-type ExpISCC1, specific for Pectobacterium carotovorum subsp. carotovorum 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + 3-oxooctanoyl-[acyl-carrier protein] substrate of wild-type ExpISCC3065, specific for Pectobacterium carotovorum subsp. carotovorum 5'-methylthioadenosine + N-3-oxooctanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] substrate of wild-type ExpISCC1, very low activity Pectobacterium carotovorum subsp. carotovorum 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] substrate of wild-type ExpISCC3065, very low activity Pectobacterium carotovorum subsp. carotovorum 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.184 acylhomoserine lactone synthase
-
 Pectobacterium carotovorum subsp. carotovorum
2.3.1.184 AHSL synthase
-
 Pectobacterium carotovorum subsp. carotovorum
2.3.1.184 ExpISCC1
-
 Pectobacterium carotovorum subsp. carotovorum
2.3.1.184 ExpISCC3065
-
 Pectobacterium carotovorum subsp. carotovorum