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Literature summary extracted from

  • Tronchere, H.; Laporte, J.; Pendaries, C.; Chaussade, C.; Liaubet, L.; Pirola, L.; Mandel, J.L.; Payrastre, B.
    Production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells (2004), J. Biol. Chem., 279, 7304-7312.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.95 medicine mutations in the MTM1 gene are associated with theX-linked myotubular myopathy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.64 expression of GST-tagged enzyme in Escherichia coli strain DH5alpha, overexpression in COS-7 cells, HEK-293 cells, L6 cells, C2C12 cells, and Jurkat with 10-15% expression level in all cases Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.64 D278A site-directed mutagenesis, inactive, substrate-trapping mutant Homo sapiens
3.1.3.95 C375S complete loss of activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.64 additional information Homo sapiens enzyme deficiency is involved in XLMTM myopathies ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.64 Homo sapiens Q13496
-
-
3.1.3.95 Homo sapiens Q13496 isoform MTM1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.64 recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.64 fibroblast
-
 Homo sapiens
-
3.1.3.64 myoblast
-
 Homo sapiens
-
3.1.3.64 myotube fibroblast cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.64 1-hexanoyl-2-lysophosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-hexanoyl-2-lysophosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.64 hexanoylglyceryl 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O
-
 Homo sapiens hexanoylglyceryl 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.64 additional information enzyme deficiency is involved in XLMTM myopathies Homo sapiens ?
-
 ?
3.1.3.64 additional information the enzyme shows specificity for 1-phosphatidyl-1D-myo-inositol 3-phosphate and 1-phosphatidyl-1D-myo-inositol 3,3-bisphosphate independent of the side acyl chain length Homo sapiens ?
-
 ?
3.1.3.95 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate isoform MTM1 can hydrolyze phosphatidylinositol 3,5-bisphosphate both in vitro and in mammalian cells ?
3.1.3.95 additional information isoform MTM1 additionally hydrolyzes 1-phosphatidyl-1D-myo-inositol 3-phosphate, reaction of EC 3.1.3.64 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.64 MTM1
-
 Homo sapiens
3.1.3.64 myotubularin
-
 Homo sapiens
3.1.3.64 phosphoinositide 3-phosphatase
-
 Homo sapiens
3.1.3.64 PtdIns(3)P phosphatase
-
 Homo sapiens
3.1.3.95 MTM1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.64 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.64 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.3.95 physiological function in L6 myotubes overexpressing isoform MTM1, hyperosmotic shock induces an increase in the mass level of 1-phosphatidyl-1D-myo-inositol 5-phosphate that is reduced by 50% upon overexpression of the MTM1 inactive mutant D278A Homo sapiens