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Literature summary extracted from

  • Pell, G.; Szabo, L.; Charnock, S.J.; Xie, H.; Gloster, T.M.; Davies, G.J.; Gilbert, H.J.
    Structural and biochemical analysis of Cellvibrio japonicus xylanase 10C: how variation in substrate-binding cleft influences the catalytic profile of family GH-10 xylanases (2004), J. Biol. Chem., 279, 11777-11788.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.8
-
 Cellvibrio japonicus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 F181Y isoform xylanase 10A, enzymic activity similar to wild-type Cellvibrio japonicus
3.2.1.8 G295E isoform xylanase 10C, enzymic activity similar to wild-type Cellvibrio japonicus
3.2.1.8 Y340A isoform xylanase 10C, enzymic activity reduced by more than 90% Cellvibrio japonicus
3.2.1.8 Y434F isoform xylanase 10C, enzymic activity similar to wild-type Cellvibrio japonicus
3.2.1.8 Y87A isoform xylanase 10A, reduced enzymic activity Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Cellvibrio japonicus
-
 isoforms xylanase 10c, xylanase 10A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl 4-O-beta-D-xylopyranosyl-beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus 4-nitrophenol + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
-
 ?
3.2.1.8 4-nitrophenyl beta-D-cellobioside + H2O
-
 Cellvibrio japonicus 4-nitrophenol + beta-D-cellobiose
-
 ?
3.2.1.8 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.8 arabinoxylan + H2O from wheat or rye Cellvibrio japonicus ?
-
 ?
3.2.1.8 glucuronoxylan + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.2.1.8 additional information very low activity against xylooligosaccharides Cellvibrio japonicus ?
-
 ?
3.2.1.8 xylan + H2O from oatspelt Cellvibrio japonicus ?
-
 ?
3.2.1.8 xylohexaose + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.2.1.8 xylotetraose + H2O
-
 Cellvibrio japonicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 More bi-modular enzyme comprising a GH10 catalytic module and a family 15 carbohydrate-binding module, crystallization data Cellvibrio japonicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase 10A
-
 Cellvibrio japonicus
3.2.1.8 xylanase 10C
-
 Cellvibrio japonicus