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Literature summary extracted from

  • Rudrabhatla, P.; Rajasekharan, R.
    Mutational analysis of stress-responsive peanut dual specificity protein kinase. Identification of tyrosine residues involved in regulation of protein kinase activity (2003), J. Biol. Chem., 278, 17328-17335.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.12.1 additional information the enzyme is induced by abiotic stresses, the activation loop is located between subdomains VII and VIII, phosphorylation at Tyr317, Tyr297, and Tyr148 activates the enzyme Arachis hypogaea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.12.1 overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Arachis hypogaea

Protein Variants

EC Number Protein Variants Comment Organism
2.7.12.1 Y148F site-directed mutagenesis, ATP binding site residue, the mutant is unable to phosphorylate histone and is not phosphorylated itself Arachis hypogaea
2.7.12.1 Y213F site-directed mutagenesis, TEY motif residue, the mutant shows 4fold increased autophosphorylation and 2.8fold increased activity with ATP and histone compared to the wild-type enzyme Arachis hypogaea
2.7.12.1 Y297F site-directed mutagenesis, inactive mutant enzyme Arachis hypogaea
2.7.12.1 Y317F site-directed mutagenesis, ATP binding site residue, the mutant is unable to phosphorylate histone and is not phosphorylated itself Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.12.1 0.006
-
 histone H1 recombinant mutant Y213F Arachis hypogaea
2.7.12.1 0.007
-
 histone H1 recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.009
-
 histone H1 recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.011
-
 histone H1 recombinant mutants Y148F and Y297F Arachis hypogaea
2.7.12.1 0.012
-
 histone H1 recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.019
-
 ATP recombinant mutant Y213F Arachis hypogaea
2.7.12.1 0.034
-
 ATP recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.059
-
 ATP recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.081
-
 ATP recombinant mutant Y297F Arachis hypogaea
2.7.12.1 0.107
-
 ATP recombinant mutant Y148F Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.12.1 additional information Arachis hypogaea the enzyme is developmentally regulated ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.12.1 Arachis hypogaea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.12.1 phosphoprotein MALDI-MS phosphorylation site mapping, the enzyme performs autophosphorylation within the ATP binding site at Tyr148 and Tyr317, at Tyr213 of the activation loop, and at Tyr297 in the C-terminus Arachis hypogaea

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.12.1 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.12.1 ATP + histone H1
-
 Arachis hypogaea ADP + phosphorylated histone H1
-
 ?
2.7.12.1 additional information the enzyme is developmentally regulated Arachis hypogaea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.12.1 More analysis of sequence motifs Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
2.7.12.1 serine/threonine/tyrosine protein kinase
-
 Arachis hypogaea
2.7.12.1 STY protein kinase
-
 Arachis hypogaea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.12.1 0.26
-
 ATP recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.54
-
 ATP recombinant mutant Y297F Arachis hypogaea
2.7.12.1 1.02
-
 ATP recombinant mutant Y148F Arachis hypogaea
2.7.12.1 3.09
-
 histone H1 recombinant mutant Y148F Arachis hypogaea
2.7.12.1 3.3
-
 histone H1 recombinant mutant Y317F Arachis hypogaea
2.7.12.1 3.71
-
 histone H1 recombinant mutant Y297F Arachis hypogaea
2.7.12.1 4.74
-
 histone H1 recombinant mutant Y317F Arachis hypogaea
2.7.12.1 5.25
-
 ATP recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 14.5
-
 ATP recombinant mutant Y213F Arachis hypogaea
2.7.12.1 18.4
-
 histone H1 recombinant mutant Y213F Arachis hypogaea

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.12.1 ATP binding site residue Tyr317 is important Arachis hypogaea