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Literature summary extracted from

  • SooHoo, C.K.; Hollocher, T.C.
    Purification and characterization of nitrous oxide reductase from Pseudomonas aeruginosa strain P2 (1991), J. Biol. Chem., 266, 2203-2209.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.4 0.0024
-
 N2O pH 7.1, 25°C Pseudomonas aeruginosa
1.7.2.4 0.0043
-
 benzyl viologen cation radical pH 7.1, 25°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu 4 Cu atoms/subunit Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.4 73000
-
 2 * 73000, SDS-PAGE Pseudomonas aeruginosa
1.7.2.4 120000
-
 HPLC gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Pseudomonas aeruginosa
-
-
-
1.7.2.4 Pseudomonas aeruginosa P2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 additional information
-
 Vmax N2O 0.2 - 0.5 micromol/min/mg, pH 7.1, 25°C Pseudomonas aeruginosa
1.7.2.4 27
-
 pH 7.1, 25°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 N2O + H2O + benzyl viologen cation radical
-
 Pseudomonas aeruginosa N2 + reduced benzyl viologen
-
 ?
1.7.2.4 N2O + H2O + benzyl viologen cation radical
-
 Pseudomonas aeruginosa P2 N2 + reduced benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 dimer 2 * 73000, SDS-PAGE Pseudomonas aeruginosa