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Literature summary extracted from

  • Woodson, J.D.; Escalante-Semerena, J.C.
    The cbiS gene of the archaeon Methanopyrus kandleri AV19 encodes a bifunctional enzyme with adenosylcobinamide amidohydrolase and alpha-ribazole-phosphate phosphatase activities (2006), J. Bacteriol., 188, 4227-4235.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.90 MgCl2 activity is enhanced 2.7fold by adding 10 mM MgCl2 to the reaction mixture Methanopyrus kandleri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.90 expression in Escherichia coli and Salmonella enterica Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.90 39000
-
 monomer, SDS-PAGE Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.90 Methanopyrus kandleri
-
 AV19
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.90
-
 Methanopyrus kandleri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.90 0.009
-
 (CN)2-cobinamide-GDP as substrate Methanopyrus kandleri
3.5.1.90 0.009
-
 cyanocobalamin as substrate Methanopyrus kandleri
3.5.1.90 0.02
-
 adenosylcobinamide and (CN)2-cobinamide as substrate Methanopyrus kandleri
3.5.1.90 0.027
-
 (CN)2-cobinamide-P as substrate Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.90 (CN)2-cobinamide + H2O
-
 Methanopyrus kandleri adenosylcobyric acid + ?
-
 ?
3.5.1.90 (CN)2-cobinamide-GDP + H2O
-
 Methanopyrus kandleri adenosylcobyric acid + ?
-
 ?
3.5.1.90 (CN)2-cobinamide-P + H2O
-
 Methanopyrus kandleri adenosylcobyric acid + ?
-
 ?
3.5.1.90 adenosylcobinamide + H2O pH 9.0, anaerobical incubation at 90°C for 1 h Methanopyrus kandleri adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
3.5.1.90 cyanocobalamin + H2O
-
 Methanopyrus kandleri adenosylcobyric acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.90 adenosylcobinamide amidohydrolase
-
 Methanopyrus kandleri
3.5.1.90 AdoCbi amidohydrolase
-
 Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.90 90 100
-
 Methanopyrus kandleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.90 9
-
-
 Methanopyrus kandleri