Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miyake, O.; Ochiai, A.; Hashimoto, W.; Murata, K.
    Origin and diversity of alginate lyases of families PL-5 and -7 in Sphingomonas sp. strain A1 (2004), J. Bacteriol., 186, 2891-2896.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression of native A1-II' and a truncated A1-II' lacking the N-terminal 80 amino acid residues in Escherichia coli Sphingomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Hg2+ 1 mM, 30% inhibition Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 25000
-
 gel filtration Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Sphingomonas sp. Q75WP3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant Sphingomonas sp.

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 25000, SDS-PAGE Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase A1-II'
-
 Sphingomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 40
-
-
 Sphingomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 30 50 30°C: about 55% of maximal activity, 50°C: about 70% of maximal activity Sphingomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 30
-
 pH 7.5, 5 min, stable Sphingomonas sp.
4.2.2.3 35
-
 pH 7.5, 5 min, about 10% loss of initial activity Sphingomonas sp.
4.2.2.3 40
-
 pH 7.5, 5 min, about 35% loss of initial activity Sphingomonas sp.
4.2.2.3 45
-
 pH 7.5, 5 min, about 50% loss of initial activity Sphingomonas sp.
4.2.2.3 50
-
 pH 7.5, 5 min, about 65% loss of initial activity Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.5
-
-
 Sphingomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 6 8.8 pH 6.0: about 45% of maximal activity, pH 8.8: about 50% of maximal activity Sphingomonas sp.