Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sugano, Y.; Kodama, H.; Terada, I.; Yamazaki, Y.; Noma, M.
    Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107 (1994), J. Bacteriol., 176, 6812-6818.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.159 5.37
-
 neoagarobiose pH 7.7, 30°C Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.159 42000
-
 2 * 42000, SDS-PAGE Vibrio sp.
3.2.1.159 84000
-
 gel filtration Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.159 Vibrio sp.
-
-
-
3.2.1.159 Vibrio sp. JT0107
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.159
-
 Vibrio sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.159 92
-
 pH 7.7, 30°C Vibrio sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.159 -20°C, phosphate buffer pH 7.8, 30% glycerol, 2 months, 60% residual activity Vibrio sp.
3.2.1.159 4°C, phosphate buffer pH 7.8, 3 weeks, 60% residual activity, 2 months, 30% residual activity Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.159 alpha-1,3-neoagarooligosaccharide + H2O
-
 Vibrio sp. agaropentaose + agarotriose + agarobiose + D-galactose
-
 ?
3.2.1.159 alpha-1,3-neoagarooligosaccharide + H2O
-
 Vibrio sp. JT0107 agaropentaose + agarotriose + agarobiose + D-galactose
-
 ?
3.2.1.159 neoagarobiose + H2O
-
 Vibrio sp. ?
-
 ?
3.2.1.159 neoagarobiose + H2O
-
 Vibrio sp. JT0107 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.159 dimer 2 * 42000, SDS-PAGE Vibrio sp.
3.2.1.159 More N-terminal amino acid sequence Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.159 30
-
-
 Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.159 30
-
 120 min, stable Vibrio sp.
3.2.1.159 40
-
 rapid loss of activity Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.159 7.7
-
-
 Vibrio sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.159 6.2 8.9 drastical reduction in activity below or above Vibrio sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.159 Vibrio sp. isoelectric focusing
-
 4.6