Any feedback?
Literature summary extracted from
- Characterization of a thermostable recombinant beta-galactosidase from Thermotoga maritima (2004), J. Appl. Microbiol., 97, 1006-1014.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | expression in Escherichia coli | Thermotoga maritima |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.23 | additional information | - |
additional information | enzyme displays non-Michaelis-Menten reaction kinetics with substrate activation for lactose, which is explained by simultaneous reactions of hydrolysis and transgalactosylation | Thermotoga maritima | |
| 3.2.1.23 | 0.33 | - |
o-nitrophenyl-beta-D-galactoside | 80°C | Thermotoga maritima |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.23 | Mn2+ | most effective divalent cation for beta-galactosidase activity on both o-nitrophenyl-beta-D-galactoside and lactose | Thermotoga maritima | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 129501 | - |
x * 129501, calculated from sequence | Thermotoga maritima |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | - |
Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.23 | lactose + H2O | - |
Thermotoga maritima | D-glucose + D-galactose | - |
? | |
| 3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermotoga maritima | o-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | ? | x * 129501, calculated from sequence | Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 80 | - |
hydrolysis of lactose | Thermotoga maritima |
| 3.2.1.23 | 85 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Thermotoga maritima |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 80 | - |
half-life: 16 h | Thermotoga maritima |
| 3.2.1.23 | 90 | - |
half-life: 16 min | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 6.5 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside and lactose | Thermotoga maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
