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Literature summary extracted from

  • Chang, S.W.; Lee, G.C.; Shaw, J.F.
    Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase (2006), J. Agric. Food Chem., 54, 815-822.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 additional information codon optimization for improved expression of LIP1 in Pichia pastoris for uuse of the enzyme in industrial processes Diutina rugosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lip1, expression in Pichia pastoris, culture condition and codon optimization for improved expression, secretion of the recombinant enzyme, overview Diutina rugosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information construction of a regional synthetic lip1 gene for improved expression in Pichia pastoris, overview Diutina rugosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 additional information no inhibition by Tween 80, Brij35, Triton X-100, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, and sodium taurocholate at 0.1-1.0% w/v Diutina rugosa
3.1.1.3 SDS 98% inhibition at 0.1% w/v Diutina rugosa
3.1.1.3 Tween 20 18-19% inhibition at 0.1-1.0% w/v Diutina rugosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Diutina rugosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Diutina rugosa P20261 LIP1 precrusor; i.e. Candida cylindracea, gene lip1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant secreted enzyme 25.6fold from Pichia pastoris by hydrophobic interaction chromatography Diutina rugosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 6.4
-
 recombinant enzyme in culture medium Diutina rugosa
3.1.1.3 7200
-
 purified recombinant enzyme, substrate 4-nitrophenyl laurate Diutina rugosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Diutina rugosa 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O good substrate Diutina rugosa 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O
-
 Diutina rugosa 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O good substrate Diutina rugosa 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O best substrate Diutina rugosa 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O
-
 Diutina rugosa 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O lower activity Diutina rugosa 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 60000, recombinant enzyme, SDS-PAGE Diutina rugosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 Lip1
-
 Diutina rugosa
3.1.1.3 lipase
-
 Diutina rugosa
3.1.1.3 More the enzyme belongs to the CRL multigne family Diutina rugosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
 recombinant enzyme Diutina rugosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30 40 10 min, recombinant enzyme, pH 7.0, stable at, inactivation above Diutina rugosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
 recombinant enzyme Diutina rugosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 4 6 14 h, 95-100% remaining activity Diutina rugosa