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Literature summary extracted from

  • Nguyen, T.; Splechtna, B.; Steinboeck, M.; Kneifel, W.; Lettner, H.P.; Kulbe, K.D.; Haltrich, D.
    Purification and characterization of two novel beta-galactosidases from Lactobacillus reuteri (2006), J. Agric. Food Chem., 54, 4989-4998.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol 1 mM, slight activation (6%), strain L461 Limosilactobacillus reuteri
3.2.1.23 2-mercaptoethanol 1 mM, slight activation (9%), strain L103 Limosilactobacillus reuteri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 1,4-dithiothreitol 10 mM, 41% inhibition, strain L103; 10 mM, 58% inhibition, strain L461 Limosilactobacillus reuteri
3.2.1.23 2-mercaptoethanol 10 mM, 22% inhibition, strain 461 Limosilactobacillus reuteri
3.2.1.23 Ca2+ 1 mM in presence of 10 mM Na+, 30% inhibition, strain L461 Limosilactobacillus reuteri
3.2.1.23 Cu2+ 10 mM Cu2+ in presence of 10 mM Na+, complete inhibition, strain L103; 10 mM Cu2+ in presence of 10 mM Na+, complete inhibition, strain L461 Limosilactobacillus reuteri
3.2.1.23 D-galactose strain L103; strain L461 Limosilactobacillus reuteri
3.2.1.23 EDTA 0.1 mM, 85% inhibition, strain L461; 0.1 mM, 90% inhibition, strain L103 Limosilactobacillus reuteri
3.2.1.23 Fe2+ 1 mM in presence of 10 mM Na+, 30% inhibition, strain L461 Limosilactobacillus reuteri
3.2.1.23 glucose strain L461 Limosilactobacillus reuteri
3.2.1.23 Urea 10 mM, 10% inhibition, strain L103; 10 mM, 11% inhibition, strain L461 Limosilactobacillus reuteri
3.2.1.23 Zn2+ 10 mM Cu2+ in presence of 10 mM Na+, complete inhibition, strain L461 Limosilactobacillus reuteri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.98
-
 o-nitrophenyl-beta-D-galactoside pH 6.5, 30°C, strain L461 Limosilactobacillus reuteri
3.2.1.23 31
-
 lactose pH 6.5, 30°C, strain L461 Limosilactobacillus reuteri
3.2.1.23 60
-
 lactose pH 6.5, 30°C, strain L103 Limosilactobacillus reuteri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.23 intracellular
-
 Limosilactobacillus reuteri 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 K+ either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L461 Limosilactobacillus reuteri
3.2.1.23 Na+ either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 Na+ either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L461 Limosilactobacillus reuteri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 35000
-
 1 * 35000 + 1 * 72000, strain L103, SDS-PAGE Limosilactobacillus reuteri
3.2.1.23 35000
-
 1 * 35000 + 1 * 72000, strain L461, SDS-PAGE Limosilactobacillus reuteri
3.2.1.23 72000
-
 1 * 35000 + 1 * 72000, strain L103, SDS-PAGE Limosilactobacillus reuteri
3.2.1.23 72000
-
 1 * 35000 + 1 * 72000, strain L461, SDS-PAGE Limosilactobacillus reuteri
3.2.1.23 105000
-
 strain L103, non-denaturing PAGE Limosilactobacillus reuteri
3.2.1.23 105000
-
 strain L461, non-denaturing PAGE Limosilactobacillus reuteri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Limosilactobacillus reuteri
-
-
-
3.2.1.23 Limosilactobacillus reuteri L103
-
-
-
3.2.1.23 Limosilactobacillus reuteri L461
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Limosilactobacillus reuteri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 158
-
 strain L103 Limosilactobacillus reuteri
3.2.1.23 180
-
 strain L461 Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 4-methylumbelliferyl beta-D-galactoside + H2O
-
 Limosilactobacillus reuteri 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.23 4-methylumbelliferyl beta-D-galactoside + H2O
-
 Limosilactobacillus reuteri L461 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.23 4-methylumbelliferyl beta-D-galactoside + H2O
-
 Limosilactobacillus reuteri L103 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Limosilactobacillus reuteri D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Limosilactobacillus reuteri L461 D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Limosilactobacillus reuteri L103 D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Limosilactobacillus reuteri o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Limosilactobacillus reuteri L461 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Limosilactobacillus reuteri L103 o-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 1 * 35000 + 1 * 72000, strain L103, SDS-PAGE Limosilactobacillus reuteri
3.2.1.23 dimer 1 * 35000 + 1 * 72000, strain L461, SDS-PAGE Limosilactobacillus reuteri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 45
-
 hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside, strain L461 Limosilactobacillus reuteri
3.2.1.23 45
-
 hydrolysis of lactose, strain L103 Limosilactobacillus reuteri
3.2.1.23 50
-
 hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 Limosilactobacillus reuteri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 20 60 20°C: about 55% of maximal activity, 55°C: about 30% of maximal activity, hydrolysis of lactose, strain L461 Limosilactobacillus reuteri
3.2.1.23 20 55 20°C: about 40% of maximal activity, 55°C: about 50% of maximal activity, hydrolysis of lactose, strain L103 Limosilactobacillus reuteri
3.2.1.23 30 60 30°C: about 40% of maximal activity, 60°C: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 Limosilactobacillus reuteri
3.2.1.23 35 60 35°C: about 55% of maximal activity, 60°C: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461 Limosilactobacillus reuteri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 additional information
-
 thermal stability is improved in presence of MgCl2, strain L103 Limosilactobacillus reuteri
3.2.1.23 additional information
-
 thermal stability is improved in presence of MgCl2, strain L461 Limosilactobacillus reuteri
3.2.1.23 25
-
 pH 6.0, 48 h, about 20% loss of activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 25
-
 pH 6.5, 48 h, stable below, strain L461 Limosilactobacillus reuteri
3.2.1.23 30
-
 pH 6.0, 48 h, about 40% loss of activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 30
-
 pH 6.5, 48 h, about 30% loss of activity, strain L461 Limosilactobacillus reuteri
3.2.1.23 37
-
 pH 6.0, 48 h, about 70% loss of activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 37
-
 pH 6.5, 48 h, about 50% loss of activity, strain L461 Limosilactobacillus reuteri
3.2.1.23 45
-
 pH 6.0, 48 h, complete loss of activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 45
-
 pH 6.5, 48 h, complete loss of activity, strain L461 Limosilactobacillus reuteri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 13
-
 lactose pH 6.5, 30°C, strain L103 Limosilactobacillus reuteri
3.2.1.23 58
-
 lactose pH 6.5, 30°C, strain L461 Limosilactobacillus reuteri
3.2.1.23 338
-
 o-nitrophenyl-beta-D-galactoside pH 6.5, 30°C, strain L461 Limosilactobacillus reuteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
 hydrolysis of lactose, strain L461 Limosilactobacillus reuteri
3.2.1.23 8
-
 hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside, strain L103 Limosilactobacillus reuteri
3.2.1.23 8
-
 hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461 Limosilactobacillus reuteri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 5.5 8.8 pH 5.5: about 50% of maximal activity, pH 8.8: about 60% of maximal activity, hydrolysis of lactose, strain L461 Limosilactobacillus reuteri
3.2.1.23 6 9 pH 6.0: about 40% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of lactose, strain L103 Limosilactobacillus reuteri
3.2.1.23 6 8.5 pH 6.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 Limosilactobacillus reuteri
3.2.1.23 6.2 8.5 pH 6.2: about 60% of maximal activity, pH 8.5: about 75% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461 Limosilactobacillus reuteri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 5.5
-
 37°C, 3 h, about 60% loss of activity, strain L103 Limosilactobacillus reuteri
3.2.1.23 6
-
 37°C, 10% loss of activity after 3 h, 40% loss of activity after 20 h, strain L103 Limosilactobacillus reuteri
3.2.1.23 6 6.5 enzyme is most stable between pH 6.0 and pH 6.5, strain L461 Limosilactobacillus reuteri
3.2.1.23 7
-
 37°C, 3 h, complete inactivation, strain L103 Limosilactobacillus reuteri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 89
-
 D-galactose pH 6.5, 30°C, substrate: lactose, strain L461 Limosilactobacillus reuteri
3.2.1.23 115
-
 D-galactose pH 6.5, 30°C, substrate: o-nitrophenyl-beta-D-galactoside, strain L461 Limosilactobacillus reuteri
3.2.1.23 188
-
 D-galactose pH 6.5, 30°C, substrate: lactose, strain L103 Limosilactobacillus reuteri
3.2.1.23 683
-
 D-glucose pH 6.5, 30°C, substrate: o-nitrophenyl-beta-D-galactoside, strain L461 Limosilactobacillus reuteri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.23 Limosilactobacillus reuteri isoelectric focusing, pH-range 3-10, strain L461
-
 3.84
3.2.1.23 Limosilactobacillus reuteri isoelectric focusing, pH-range 3-10, strain L103 4.8 4.6