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Literature summary extracted from

  • Sio, C.F.; Otten, L.G.; Cool, R.H.; Diggle, S.P.; Braun, P.G.; Bos, R.; Daykin, M.; Camara, M.; Williams, P.; Quax, W.J.
    Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1 (2006), Infect. Immun., 74, 1673-1682.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.97 biotechnology the enzyme is useful as a biosensor Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.97 expression in Escherichia coli strain DH10B Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.97 additional information Pseudomonas aeruginosa virulence of the opportunistic human pathogen is controlled by the N-acyl-homoserine lactone, AHL-dependent quorum-sensing system, the enzyme inactivates AHLs by hydrolysis of the carboxylic side chain with side chain ranging from 11 to 14 C-atoms with broad range specificity, addition of the purified enzyme to cell cultures results in complete inhibition of N-3-oxododecanoyl-(S)-homoserine lactone accumulation and production of the signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone, as well as reduced production of virulence factors elastase and procyanin ?
-
 ?
3.5.1.97 N-3-oxododecanoyl-(S)-homoserine lactone + H2O Pseudomonas aeruginosa
-
 (S)-homoserine + 3-oxododecanoic acid
-
 ?
3.5.1.97 N-dodecanoyl-(S)-homoserine lactone + H2O Pseudomonas aeruginosa
-
 (S)-homoserine + dodecanoic acid
-
 ?
3.5.1.97 N-tetradecanoyl-(S)-homoserine lactone + H2O Pseudomonas aeruginosa
-
 (S)-homoserine + tetradecanoic acid
-
 ?
3.5.1.97 N-undecanoyl-(S)-homoserine lactone + H2O Pseudomonas aeruginosa
-
 (S)-homoserine + undecanoic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.97 Pseudomonas aeruginosa
-
 gene PA2385
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.97 recombinant enzyme from Escherichia coli strain DH10B by several chromatographic steps Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.97 additional information virulence of the opportunistic human pathogen is controlled by the N-acyl-homoserine lactone, AHL-dependent quorum-sensing system, the enzyme inactivates AHLs by hydrolysis of the carboxylic side chain with side chain ranging from 11 to 14 C-atoms with broad range specificity, addition of the purified enzyme to cell cultures results in complete inhibition of N-3-oxododecanoyl-(S)-homoserine lactone accumulation and production of the signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone, as well as reduced production of virulence factors elastase and procyanin Pseudomonas aeruginosa ?
-
 ?
3.5.1.97 additional information N-acyl-homoserine lactone substrate specificity, no activity with N-butanoyl-(S)-homoserine lactone, N-hexanoyl-(S)-homoserine lactone, and N-3-oxohexanoyl-(S)-homoserine lactone, overview Pseudomonas aeruginosa ?
-
 ?
3.5.1.97 N-3-oxodecanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine lactone + 3-oxodecanoic acid
-
 ?
3.5.1.97 N-3-oxododecanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine + 3-oxododecanoic acid
-
 ?
3.5.1.97 N-dodecanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine + dodecanoic acid
-
 ?
3.5.1.97 N-heptanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine + heptanoic acid
-
 ?
3.5.1.97 N-octanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine + octanoic acid
-
 ?
3.5.1.97 N-tetradecanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine + tetradecanoic acid
-
 ?
3.5.1.97 N-undecanoyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine + undecanoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.97 More the enzyme belongs to the N-terminal nucleophile hydrolase superfamily Pseudomonas aeruginosa
3.5.1.97 N-acyl-homoserine lactone acylase
-
 Pseudomonas aeruginosa
3.5.1.97 PA2385 protein
-
 Pseudomonas aeruginosa
3.5.1.97 quorum-quenching AHL acylase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.97 30
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.97 7.4
-
 assay at Pseudomonas aeruginosa