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Literature summary extracted from

  • Fundoiano-Hershcovitz, Y.; Rabinovitch, L.; Shulami, S.; Reiland, V.; Shoham, G.; Shoham, Y.
    The ywad gene from Bacillus subtilis encodes a double-zinc aminopeptidase (2005), FEMS Microbiol. Lett., 243, 157-163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 gene ywad, overexpression in Escherichia coli Bacillus subtilis
3.4.11.10 gene ywad, overexpression in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline complete inhibition, reversible by Zn2+ Bacillus subtilis
3.4.11.10 1,10-phenanthroline complete inhibition, reversible by Zn2+ Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 additional information
-
 additional information kinetics Bacillus subtilis
3.4.11.6 0.6
-
 L-Arg-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 0.85
-
 L-Lys-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 3.6
-
 L-Leu-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 10
-
 L-Ala-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 19
-
 L-Val-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 additional information
-
 additional information kinetics Bacillus subtilis
3.4.11.10 0.6
-
 L-Arg-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 0.85
-
 L-Lys-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 3.6
-
 L-Leu-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 10
-
 L-Ala-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 19
-
 L-Val-4-nitroanilide pH 8.0, 30°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Zn2+ zinc-metalloaminopeptidase, 2 Zn2+ per enzyme molecule Bacillus subtilis
3.4.11.10 Zn2+ zinc-metalloaminopeptidase, 2 Zn2+ per enzyme molecule Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Bacillus subtilis P25152 ywad gene
-
3.4.11.10 Bacillus subtilis P25152 ywad gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 recombinant enzyme 5fold from Escherichia coli by ion exchange chromatography Bacillus subtilis
3.4.11.10 recombinant enzyme 5fold from Escherichia coli by ion exchange chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.6 additional information
-
-
 Bacillus subtilis
3.4.11.6 64
-
 purified recombinant enzyme Bacillus subtilis
3.4.11.10 additional information
-
-
 Bacillus subtilis
3.4.11.10 64
-
 purified recombinant enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 L-Ala-4-nitroanilide + H2O low activity Bacillus subtilis L-Ala + 4-nitroaniline
-
 ?
3.4.11.6 L-Arg-4-nitroanilide + H2O best substrate Bacillus subtilis L-Arg + 4-nitroaniline
-
 ?
3.4.11.6 L-Leu-4-nitroanilide + H2O
-
 Bacillus subtilis L-Leu + 4-nitroaniline
-
 ?
3.4.11.6 L-Lys-4-nitroanilide + H2O
-
 Bacillus subtilis L-Lys + 4-nitroaniline
-
 ?
3.4.11.6 L-Val-4-nitroanilide + H2O low activity Bacillus subtilis L-Val + 4-nitroaniline
-
 ?
3.4.11.6 additional information substrate specificity, 4-nitroanilides of Gly, Glu, Asp, Phe, Pro, and Ala-Pro are poor substrates, overview Bacillus subtilis ?
-
 ?
3.4.11.10 L-Ala-4-nitroanilide + H2O low activity Bacillus subtilis L-Ala + 4-nitroaniline
-
 ?
3.4.11.10 L-Arg-4-nitroanilide + H2O best substrate Bacillus subtilis L-Arg + 4-nitroaniline
-
 ?
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Bacillus subtilis L-Leu + 4-nitroaniline
-
 ?
3.4.11.10 L-Lys-4-nitroanilide + H2O
-
 Bacillus subtilis L-Lys + 4-nitroaniline
-
 ?
3.4.11.10 L-Val-4-nitroanilide + H2O low activity Bacillus subtilis L-Val + 4-nitroaniline
-
 ?
3.4.11.10 additional information substrate specificity, 4-nitroanilides of Gly, Glu, Asp, Phe, Pro, and Ala-Pro are poor substrates, overview Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 BSAP
-
 Bacillus subtilis
3.4.11.6 double-zinc aminopeptidase
-
 Bacillus subtilis
3.4.11.10 BSAP
-
 Bacillus subtilis
3.4.11.10 double-zinc aminopeptidase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.6 30
-
 assay at Bacillus subtilis
3.4.11.10 30
-
 assay at Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.6 80
-
 20 min, purified recombinant enzyme, stable Bacillus subtilis
3.4.11.10 80
-
 20 min, purified recombinant enzyme, stable Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.6 0.37
-
 L-Ala-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 0.43
-
 L-Val-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 10
-
 L-Lys-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 34
-
 L-Arg-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.6 55
-
 L-Leu-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 0.37
-
 L-Ala-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 0.43
-
 L-Val-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 10
-
 L-Lys-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 34
-
 L-Arg-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.4.11.10 55
-
 L-Leu-4-nitroanilide pH 8.0, 30°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 8.4
-
-
 Bacillus subtilis
3.4.11.10 8.4
-
-
 Bacillus subtilis